# Novel Multi-Depth Two-Photon Microscope for Measuring Neuronal Network Plasticity

> **NIH NIH R21** · UNIVERSITY OF CALIFORNIA, SAN DIEGO · 2021 · $89,681

## Abstract

PROJECT SUMMARY
 Decades of studies in both human subjects and animal models indicate that the hippocampus is a critical
brain structure involved in both the formation of memories and spatial navigation. Both of these computations
are thought to create a cognitive map of features within our environment, which often contains dynamic sensory
features that continuously change with time. However, it is not known if these seemingly separate computations
within the hippocampus are interdependent, recruit common circuits, or utilize similar encoding strategies. In the
parent R21 Trailblazer Award, we proposed to build a novel microscope that is capable of imaging neuronal
activity in multiple hippocampal subfields simultaneously in order to ask how the hippocampus learns dynamic
information by studying the formation of musical memories in mice. In the diversity supplement, the candidate
Cristian Sharp plans to capitalize on this microscope technology to study the role of the hippocampus and its
different subfields in navigation of spatial environments containing dynamic sensory information.
 Cristian’s goal is to become a neuroscientist, working on cutting edge neurotechnology and designing
rigorous experiments to answer fundamental questions about brain function. As part of the current diversity
supplement we have devised a detailed career development plan that will facilitate Cristian’s transition from the
field of physics to biology and prepare him for graduate school in neuroscience by 1) working with an experienced
multidisciplinary team of mentors, which include Drs. Matthew Shtrahman, Brenda Bloodgood, and David
Kleinfeld; 2) following an individualized learning plan, mastering core concepts in biology through formal
coursework; 3) performing hypothesis-focused biological research; 4) learning to communicate about his
research through scientific lectures, manuscripts, and grant applications; 5) attending professional development
courses and research conferences; and 6) preparing and submitting an NSF Graduate Research Fellowship
application during the award period. With the support of the NIH diversity supplement and by achieving these
career development milestones, Cristian will be well-poised for graduate school and his future research career
in neuroscience.

## Key facts

- **NIH application ID:** 10429581
- **Project number:** 3R21EB029139-01A1S1
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN DIEGO
- **Principal Investigator:** Matthew Shtrahman
- **Activity code:** R21 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $89,681
- **Award type:** 3
- **Project period:** 2021-09-05 → 2022-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10429581

## Citation

> US National Institutes of Health, RePORTER application 10429581, Novel Multi-Depth Two-Photon Microscope for Measuring Neuronal Network Plasticity (3R21EB029139-01A1S1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10429581. Licensed CC0.

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