# Enhancer Codes with Ligand Mediated Gene Regulation and Chromatin Architecture

> **NIH NIH K01** · GEORGETOWN UNIVERSITY · 2021 · $55,976

## Abstract

Contact PD/PI: Nair, Sreejith Janardhanan
PROJECT SUMMARY
The central objective of this research proposal is to gain novel insights into the cell biological principles
underlying the regulation of gene expression in response to hormonal stimulation. My research focus is gene
regulation mediated by transcriptional enhancers. Although recognized as the major organizer of gene
regulation in metazoans, the precise mechanism with which enhancer complex assembled and regulate target
genes in response to stimuli is still mostly not known. Preliminary data described in this proposal suggest that
acutely assembled enhancers harbor ribonucleoprotein complex with a physicochemical property that is quite
distinct from that of enhancers that are constitutively active. These enhancers get optimally activated once
associated with inter-chromatin granules (ICGs). This proposal will link multiple experimental strategies from
disciplines as diverse as molecular biology, biophysics, genomics, proteomics and microscopy to provide a
comprehensive understanding of the underlying mechanism of inducible enhancer activation and chromosomal
architecture. Together, this approach can significantly alter our current concepts about regulated gene
transcriptional programs. Importantly, the finding and experimental skills acquired from this study will also allow
the transition to an academic career as an independent investigator studying the biophysical and molecular
mechanisms of ligand regulated transcriptional program using proteomic, bioinformatics and genomic
approaches.
Page 6
Project Summary/Abstract

## Key facts

- **NIH application ID:** 10435941
- **Project number:** 3K01DK121871-03S1
- **Recipient organization:** GEORGETOWN UNIVERSITY
- **Principal Investigator:** Sreejith Janardhanan Nair
- **Activity code:** K01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $55,976
- **Award type:** 3
- **Project period:** 2019-07-01 → 2023-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10435941

## Citation

> US National Institutes of Health, RePORTER application 10435941, Enhancer Codes with Ligand Mediated Gene Regulation and Chromatin Architecture (3K01DK121871-03S1). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10435941. Licensed CC0.

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