# Development of methods for multi-omic analysis of DNA methylation and chromatin architecture in single cells

> **NIH NIH R01** · SALK INSTITUTE FOR BIOLOGICAL STUDIES · 2022 · $1,064,037

## Abstract

Project Summary/Abstract 
Chromatin organization and epigenomic modifications such as cytosine methylation play instrumental roles 
in gene regulation during human development and in healthy and disease states. Although the genome is 
nearly identical in each cell in the human body, chromatin conformation and cytosine DNA methylation are 
highly dynamic across cell types and during development. Previous studies showed that chromatin looping 
can be regulated by cytosine methylation. However, our current state of knowledge of the interactions 
between chromatin organization and DNA methylation is built on analyzing cultured cells and bulk tissues. 
The interaction between cell-­type specific chromatin looping and methylation in heterogeneous tissues 
remain largely unexplored. This project will develop single-­cell multi-­omic methods to jointly analyze 
chromatin conformation and cytosine methylation from the same cell. The methods will lead to high quality 
cell-­type specific chromatin conformation maps of human tissue, and the analysis of the relationship 
between chromatin conformation and cytosine methylation. If successful, the proposed methods will greatly 
facilitate the study of gene regulation in complex human tissues and in heterogeneous diseases such as 
cancer.

## Key facts

- **NIH application ID:** 10436890
- **Project number:** 5R01HG010634-04
- **Recipient organization:** SALK INSTITUTE FOR BIOLOGICAL STUDIES
- **Principal Investigator:** Jesse R Dixon
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $1,064,037
- **Award type:** 5
- **Project period:** 2019-09-06 → 2024-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10436890

## Citation

> US National Institutes of Health, RePORTER application 10436890, Development of methods for multi-omic analysis of DNA methylation and chromatin architecture in single cells (5R01HG010634-04). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10436890. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*