# Studies on Protein Lipidation

> **NIH NIH R01** · SCRIPPS RESEARCH INSTITUTE, THE · 2022 · $286,729

## Abstract

PROJECT SUMMARY
To elucidate the antiviral mechanism of S-palmitoylated IFITMs (1, 2 and 3) in host immunity against viruses, we
propose to perform detailed biochemical, biophysical and cellular studies of these lipidated immune effectors in
this grant renewal. For biochemical and structural studies of IFITMs, we will perform computational modeling
and reconstitute site-specifically lipidated IFITM3 in vitro (Aim 1). Moreover, we have discovered direct
interactions of IFITMs with specific cellular lipids and will investigate their significance biochemically in vitro and
in mammalian cells during virus infections (Aim 2). Lastly, we will investigate S-palmitoylated IFITM interactions
with key cellular factors involved their regulation and antiviral activity (Aim 3). These studies should help
determine how IFITMs prevent virus entry and elucidate biochemical mechanisms by which site-specific
lipidation controls membrane protein structure and function in host immunity. Determining the mechanisms that
control S-palmitoylated IFITMs function is crucial for understanding host immunity and may reveal new strategies
for combatting virus infection in humans.

## Key facts

- **NIH application ID:** 10437897
- **Project number:** 5R01GM087544-12
- **Recipient organization:** SCRIPPS RESEARCH INSTITUTE, THE
- **Principal Investigator:** Howard C Hang
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $286,729
- **Award type:** 5
- **Project period:** 2010-08-01 → 2024-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10437897

## Citation

> US National Institutes of Health, RePORTER application 10437897, Studies on Protein Lipidation (5R01GM087544-12). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10437897. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*