# Metabolomics Core

> **NIH NIH P01** · H. LEE MOFFITT CANCER CTR & RES INST · 2022 · $187,710

## Abstract

CORE 3 PROJECT SUMMARY
 METABOLISM CORE
The Metabolism Core (Core #3) supports the metabolomics and proteomics experiments designed by program
project grant (PPG) investigators as integral parts of their proposed research. These large-scale molecular
data analysis strategies are used by all four projects in this PPG, which provides synergistic linkage points,
facilitated by Core #3, across projects for cross identification of metabolites and proteins that play important
roles in lung cancer biology and immunology. Core #3 members have extensive experience in cancer
metabolism, metabolomics, proteomics, liquid chromatography-mass spectrometry, gas chromatography-mass
spectrometry, collaboration with investigators, collaboration with data scientists (including members of Core
#4), and customer service. These efforts will contribute to projects from inception to completion (i.e. from
hypothesis to publication). Core #3 will support optimization of experimental design for mass spectrometry-
based omics (Aim 1), which includes implementation of previously published strategies and development of
novel methods for sample preparation, separations including gas and liquid chromatography, and mass
spectrometry to support the experiments required by PPG investigators. Datasets will be acquired with
untargeted metabolomics, discovery proteomics, analysis of post-translational modifications, and targeted
assay development for metabolites and proteins utilizing state-of-the-art separations and mass spectrometry
equipment at Moffitt Cancer Center (Aim 2). Core #3 members will contribute to quality assurance/quality
control for data acquired for each project and collaborate with PPG investigators and other PPG Cores to
produce high quality datasets and develop data analysis strategies to maximize availability and usage of omics
data across PPG Projects (Aim 3). In summary, Core #3 will make a significant contribution to the proposed
research by elucidating molecular mechanisms underlying lung tumor biology and tumor-immune cell
interactions, which may ultimately reveal potential biomarkers and novel treatment targets to be further
investigated in functional studies proposed by the PPG Projects.

## Key facts

- **NIH application ID:** 10438719
- **Project number:** 5P01CA250984-02
- **Recipient organization:** H. LEE MOFFITT CANCER CTR & RES INST
- **Principal Investigator:** John M Koomen
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $187,710
- **Award type:** 5
- **Project period:** 2021-06-25 → 2026-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10438719

## Citation

> US National Institutes of Health, RePORTER application 10438719, Metabolomics Core (5P01CA250984-02). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10438719. Licensed CC0.

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