# Cell  Culture and Cell Phenotyping

> **NIH NIH P30** · UT SOUTHWESTERN MEDICAL CENTER · 2022 · $199,322

## Abstract

Resource/Service Core: Cell Culture and Cell Phenotyping Module
Abstract
The goal of the Cell Culture and Cell Phenotyping Module is to streamline cell culture and phenotyping
analyses for vision researchers. To accomplish this goal, this Module will support the maintenance and
authentication of cell lines that are required for rigorous study of a wide range of NEI-focused research
activities, including all NEI-funded research projects and new collaborative and pilot projects. The director and
staff of this Module will provide training, technical support, and maintenance of key equipment to facilitate
scientifically rigorous, cutting edge, vision research.
The Cell Culture arm of this module will provide the following essential services:
1) Routine maintenance of immortalized cell lines.
2) Maintenance of cryopreserved stocks of cell lines.
3) Establishment of primary corneal and retinal cell lines.
4) Routine screening for mycoplasma and endotoxin contamination.
5) Short tandem repeat DNA profiling for cell line authentication.
The Cell Phenotyping arm of this module will provide the following comprehensive analytical services:
1) Flow cytometry.
2) Cell sorting.
3) High throughput cellular analysis.
4) Live cell metabolic profiling.
5) Microvesicle quantification and size determination.
Collectively, these services will provide all NEI-funded vision researchers on campus with state of the art
resources that will increase their experimental efficiency and support the generation of high quality, quantitative
data for all aspects of vision research.

## Key facts

- **NIH application ID:** 10438809
- **Project number:** 5P30EY030413-04
- **Recipient organization:** UT SOUTHWESTERN MEDICAL CENTER
- **Principal Investigator:** DANIELLE M. ROBERTSON
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $199,322
- **Award type:** 5
- **Project period:** 2019-09-30 → 2024-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10438809

## Citation

> US National Institutes of Health, RePORTER application 10438809, Cell  Culture and Cell Phenotyping (5P30EY030413-04). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10438809. Licensed CC0.

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