OVERALL PROJECT SUMMARY This application is for renewal of the Nathan Shock Center of Excellence in the Basic Biology of Aging at the University of Washington and affiliated institutions. This Center has over the past 25 years provided key resources in support of investigators who study the biology of aging. This application continues a theme that emphasizes outreach and service to the broadest community of investigators in the gerosciences. Of proximal relevance is the characterization of aging-related phenotypes of longevity and healthspan. As our Center services must be easily accessible to outside users, our Longevity and Healthspan Core (Core E) focuses on invertebrate assays, many of them novel. Two other Resources Cores focus on the high dimensional assessments that are closely related to aging phenotypes: Protein Phenotypes of Aging (Core C) and Metabolite Phenotypes of Aging (Core D). Sophisticated computational and bioinformatic tools for data analysis and optimal insight are provided by the Artificial Intelligence and Bioinformatics Core F. Each of these four Resource Cores is led by highly respected experts in that field, including Michael MacCoss and Judit Villen (Core C), Daniel Promislow (Core D), Matt Kaeberlein and Maitreya Dunham (Core E) and Su-In Lee (Core F). Each will push the envelope of appropriate technologies, developing new state-of-the art approaches for assessments that are the most applicable to gerontology and making them accessible to the national aging community. The Research Development Core (Core B) will continue to support pilot and junior faculty studies, with a firm focus on outreach of service to the national geroscience constituency. The Administrative and Program Enrichment Core (Core A) supports administrative management, an external advisory panel, courses, and data sharing and dissemination. Core A’s program of seminars and symposia will continue a focus on sponsorship and organization of national courses, meetings and pre-meetings, as well as workshops in the fields allied to our Resource Core Services. In coordination with other Nathan Shock Centers, we will support a new Geropathology Research initiative.