# The roles of regional specialization, mechanical forces and epigenetic memory after perturbation and injury of the intestinal stem cell microenvironment

> **NIH NIH U01** · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · 2021 · $279,654

## Abstract

Project Summary
The increased complexity of methods to analyze high-throughput genomic data, particularly single cell
datasets, mandates the tight interaction between experimental and computational scientists. Analysis of how
different compartments of the intestine interact with each other and react to insults is greatly advanced by
studies at the single cell level. This project will accelerate progress of genomic analysis of the intestinal stem
cell niche by ISCC members through the following two aims, (1) collaborations on projects from individual
centers, and (2) collaboration on a consortium-wide project to determine the minimum set of genetic factors
that support the intestinal stem cell niche using a comparative approach. In the previous year of this project,
we have established collaborations with four ISCC teams to help with their project; we will now continue these
collaborations and initiate two additional collaborations. For the consortium-wide project, we will generate new
single-cell gene expression datasets to annotate a set of gene modules and communication modules in the
intestinal stem cell niche in mouse and human. This project will deliver a set of conserved and derived factors
that participate in the maintenance of the intestinal stem cell niche and a consortium-endorsed nomenclature of
all mesenchymal, epithelial, and other cell subtypes in human and mouse intestine.

## Key facts

- **NIH application ID:** 10439349
- **Project number:** 3U01DK103147-08S2
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
- **Principal Investigator:** Ophir D Klein
- **Activity code:** U01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $279,654
- **Award type:** 3
- **Project period:** 2014-09-05 → 2022-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10439349

## Citation

> US National Institutes of Health, RePORTER application 10439349, The roles of regional specialization, mechanical forces and epigenetic memory after perturbation and injury of the intestinal stem cell microenvironment (3U01DK103147-08S2). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10439349. Licensed CC0.

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