# Elucidating the regulators of ciliogenesis

> **NIH NIH R01** · NEW YORK UNIVERSITY SCHOOL OF MEDICINE · 2022 · $355,952

## Abstract

Primary cilia are antenna-like projections that emanate from the cell surface of most quiescent mammalian
cells. Cilia are essential for sensing extracellular cues and growth factors. They assemble from centrosomes
and are resorbed in a cell cycle-dependent manner. Many questions remain regarding the mechanisms that
provoke cilium assembly and disassembly. For example, links between reorganization of cytoskeletal proteins,
tubulin modifications, and nuclear anchoring have emerged, but mechanistic connections to cilium assembly
and disassembly are not well understood. We have begun characterizing key proteins required for ciliogenesis,
including Talpid3, a protein known to play a role in signaling. Using proteome-wide methods to identify Talpid3-
interaction partners, we isolated two previously uncharacterized proteins, LRRC49 and C11orf49, that play a
potent role in suppressing inappropriate cilium assembly and maintaining both tubulin glutamylation and
nuclear morphology.
In two Aims that combine cell biological and biochemical approaches with gene-editing and use of an in vivo
model, we will (1) investigate a role for LRRC49 and C11orf49 in timely cilium assembly and disassembly, (2)
examine how LRRC49 and C11orf49 regulate nuclear morphology and cytoskeleton assembly and dynamics,
and (3) test the broad biological roles of tubulin glutamylases and their connections to ciliogenesis, microtubule
stability, and nuclear envelope assembly. By investigating the function of key proteins that regulate cilium
assembly and disassembly, as well as control of cytoskeleton organization and nuclear shape in vitro and in
vivo, we will address fundamental questions related to mammalian cell growth and cell cycle progression.

## Key facts

- **NIH application ID:** 10440318
- **Project number:** 5R01GM120776-11
- **Recipient organization:** NEW YORK UNIVERSITY SCHOOL OF MEDICINE
- **Principal Investigator:** Brian D Dynlacht
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $355,952
- **Award type:** 5
- **Project period:** 2011-09-01 → 2024-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10440318

## Citation

> US National Institutes of Health, RePORTER application 10440318, Elucidating the regulators of ciliogenesis (5R01GM120776-11). Retrieved via AI Analytics 2026-05-21 from https://api.ai-analytics.org/grant/nih/10440318. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*