# Inflammatory changes and glymphatic dysfunction in carbon monoxide neuropathology

> **NIH NIH R01** · UNIVERSITY OF MARYLAND BALTIMORE · 2022 · $614,728

## Abstract

ABSTRACT
This project is to elucidate the role of inflammation and perturbations of the brain glial-based
lymphatic (glymphatic) pathway in carbon monoxide (CO)-induced brain injuries. Based on
preliminary data we hypothesize that astrocyte-derived microparticles (MPs) expressing
thrombospindin-1 play a causal role in CO-induced neuropathology that includes glymphatic
dysfunction. Further, once these MPs are liberated to the bloodstream they activate circulating
neutrophils that sequester along the neurovasculature to exacerbate inflammation, leading to
functional neurological deficits. Specific aims are: (1) Assess glymphatic function in response to
CO in mice by MRI and fluorescence microscopy; (2) Identify the role of glymphatic outflow to
CO-neuropathology in mice; (3) Determine the relationship of neuroinflammation and glymphatic
dysfunction to functional deficits; (4) Evaluate associations of morbidity in CO poisoned patients
with MPs elevations and intra-particle pro-inflammatory agents.

## Key facts

- **NIH application ID:** 10441964
- **Project number:** 1R01NS122855-01A1
- **Recipient organization:** UNIVERSITY OF MARYLAND BALTIMORE
- **Principal Investigator:** STEPHEN RAYMOND THOM
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $614,728
- **Award type:** 1
- **Project period:** 2022-03-01 → 2027-02-28

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10441964

## Citation

> US National Institutes of Health, RePORTER application 10441964, Inflammatory changes and glymphatic dysfunction in carbon monoxide neuropathology (1R01NS122855-01A1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10441964. Licensed CC0.

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