# The roles of regional specialization, mechanical forces and epigenetic memory after perturbation and injury of the intestinal stem cell microenvironment

> **NIH NIH U01** · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · 2021 · $65,412

## Abstract

PROJECT SUMMARY/ABSTRACT
The epithelium of the small intestine renews every 2 to 5 days, making it one of the most rapidly renewing
tissues in the mammalian body. This highly regenerative tissue faces a multitude of insults and injuries on a
daily basis. While much has been learned over the past few years about the intestinal stem cells (ISCs) that
fuel homeostatic renewal, the mechanisms by which the epithelium senses and responds to damage are poorly
described. Understanding how the intestine reacts to insults and how homeostasis is reestablished are
important basic science questions as well as essential starting points for translational approaches in
regenerative medicine. In the previous round of the ISC Consortium (ISCC), we identified critical signals that
regulate ISCs and discovered that injury leads to remodeling of the ISC microenvironment. In this application,
we will work toward the ISCC goal of characterizing the minimal, required factors that support ISCs in health
and disease. In the Specific Aims, we propose to determine how regional specialization, epigenetic memory
and local forces shape responses to perturbation and injury in the crypt microenvironment.

## Key facts

- **NIH application ID:** 10442012
- **Project number:** 3U01DK103147-08S1
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
- **Principal Investigator:** Ophir D Klein
- **Activity code:** U01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $65,412
- **Award type:** 3
- **Project period:** 2014-09-05 → 2022-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10442012

## Citation

> US National Institutes of Health, RePORTER application 10442012, The roles of regional specialization, mechanical forces and epigenetic memory after perturbation and injury of the intestinal stem cell microenvironment (3U01DK103147-08S1). Retrieved via AI Analytics 2026-05-21 from https://api.ai-analytics.org/grant/nih/10442012. Licensed CC0.

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