# Innovative Translational Imaging Technologies to Monitor Genome Edited Cells in Vivo

> **NIH NIH UH3** · UNIVERSITY OF CALIFORNIA AT DAVIS · 2021 · $464,044

## Abstract

PROJECT SUMMARY / ABSTRACT
This application is submitted in response to RFA-RM-18-025, which is focused on the development of new
technologies to noninvasively label and monitor genome-edited cells in vivo. Our submission meets the
objective of the RFA by establishing a new field of total-body positron emission tomography (PET) imaging for
somatic cell genome editing, and with quantitative methodologies needed to support translation. This
application is grounded in a proven infrastructure of innovative tools, technologies, and resources, and a highly
accomplished multidisciplinary team of investigators. Studies will culminate in translational tools for the in vitro
design (phantoms, quantitative measures) and in vivo methods for monitoring genome-edited cells that will
inform future clinical investigations across organ systems for the treatment of human disease. The UH2 phase
will focus on in vitro and in vivo quantitative testing with edited cells in prototype models (Specific Aim 1). The
activities in these studies can be adapted to any cell type or organ system. The UH3 phase will address
detection efficiency and safety assessments in vivo, including the potential for immune responses in an animal
model that employs quantitative imaging analysis and related tools for translation (Specific Aim 2). Overall,
these investigations will establish a new field of total-body PET imaging for monitoring the safety and efficiency
of somatic cell genome editing and will contribute new knowledge that leads to future well-designed preclinical
studies and human clinical trials.

## Key facts

- **NIH application ID:** 10447268
- **Project number:** 4UH3EB028910-03
- **Recipient organization:** UNIVERSITY OF CALIFORNIA AT DAVIS
- **Principal Investigator:** DAVID J SEGAL
- **Activity code:** UH3 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $464,044
- **Award type:** 4N
- **Project period:** 2021-08-01 → 2023-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10447268

## Citation

> US National Institutes of Health, RePORTER application 10447268, Innovative Translational Imaging Technologies to Monitor Genome Edited Cells in Vivo (4UH3EB028910-03). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10447268. Licensed CC0.

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