RNA binding proteins in end-organ autoimmune pathology

NIH RePORTER · NIH · R01 · $633,244 · view on reporter.nih.gov ↗

Abstract

IL-17 and Th17 cells are dysregulated in many pathologic auto-inflammatory conditions. Antibody-mediated glomerulonephritis (AGN) occurs when unchecked inflammation triggered by autoimmune Ab complexes that deposit in glomeruli and lead to kidney damage, which occurs in conditions such as Goodpasture disease, ANCA vasculitis, etc. Although the initiators of autoantibody-mediated pathology differ, the terminal events in end organ kidney damage have many common hallmarks, and the fundamental immunology of this process is still not well understood. Accumulating evidence from our groups and others have convincingly demonstrated role for IL-17 in driving pathogenesis of AGN in humans and in mouse models. Excessive autoimmune pathology can be caused by hyper-production of cytokines from T helper cells or by over-exuberant cytokine signaling. Therefore, in principle, molecules that influence IL-17 signal transduction have the potential to be viable targets for therapy in settings where this cytokine is a disease driver. In probing the fundamental mechanisms that mediate IL-17-dependent signaling, we identified two novel RNA binding proteins (RBPs) that contribute significantly to the pathogenesis of AGN in vivo. These RBPs are downstream of IL-17 and their activities in the IL- 17 pathway are interconnected through regulation of CCAAT Enhancer Binding Protein (C/EBP) transcription factors. In turn, C/EBPs mediate IL-17-dependent effectors that promote renal inflammation, including Lipocalin-2, neutrophil-recruiting chemokines, and feed-forward activators of Th17 differentiation such as IL-6. Our central hypothesis is that IL-17 promotes inflammation through post-transcriptional regulation of downstream mRNAs that drive renal pathology in AGN. This proposal will evaluate the molecular mechanisms by which these RBPs act and the specific physiological functions in the setting of AGN.

Key facts

NIH application ID
10450945
Project number
1R01AI162616-01A1
Recipient
UNIVERSITY OF PITTSBURGH AT PITTSBURGH
Principal Investigator
Partha Sarathi Biswas
Activity code
R01
Funding institute
NIH
Fiscal year
2022
Award amount
$633,244
Award type
1
Project period
2022-02-08 → 2027-01-31