# Vector Development Core

> **NIH NIH P01** · ST. JUDE CHILDREN'S RESEARCH HOSPITAL · 2022 · $373,111

## Abstract

PROJECT SUMMARY – CORE B
The main function of Vector Development Core (VDC) B is to develop new lentiviral vectors (LV) and the
means for their production in order to facilitate their use in clinical trials proposed in Projects 1, 2, and 3. Core
B will achieve this aim by 1) producing small-scale vectors including LV, IDLV, and AAV for preclinical testing;
2) exploring new methodologies for vector manufacturing such as transient transfection in suspension cells and
stable cell line production systems; 3) developing next generation producer cell lines from suspension cells
growing in serum-free media; 4) developing process for scale-up manufacturing that bridges laboratory
research to cGMP production; 5) optimizing protocols for LV transduction of human hematopoietic stem cells
(HSCs). As such, Core B provides a critical link between the Projects and cGMP production in Core C by
performing developmental studies, by providing the scientific expertise for process transfer, and by providing
novel and unique reagents, vectors, and cell lines for these purposes. These research activities in Core B will
ultimately integrate into all projects for improved vector manufacturing and cell transduction.

## Key facts

- **NIH application ID:** 10453803
- **Project number:** 5P01HL053749-25
- **Recipient organization:** ST. JUDE CHILDREN'S RESEARCH HOSPITAL
- **Principal Investigator:** Byoung Y Ryu
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $373,111
- **Award type:** 5
- **Project period:** 1997-09-01 → 2024-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10453803

## Citation

> US National Institutes of Health, RePORTER application 10453803, Vector Development Core (5P01HL053749-25). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10453803. Licensed CC0.

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