# Core B- Monoclonal  Antibody and Reagent Core

> **NIH NIH P01** · FEINSTEIN INSTITUTE FOR MEDICAL RESEARCH · 2022 · $295,999

## Abstract

PROJECT SUMMARY/ABSTRACT – Monoclonal Antibody and Reagent Core
This Program Project addresses the impact of antibodies cross-reactive to DNA and
the N-methyl D-aspartate receptor (NMDAR), DNRAbs, on adult and fetal brain. The
premise of the program is that DNRAbs induce a chronic inflammatory state in the adult
brain and permanent cognitive impairment in offspring exposed to these antibodies in
vitro
Projects 1 and 3 both utilize a panel of monoclonal DNRAbs. These need to be
produced in large quantity. The Monoclonal Antibody and Reagent Core will produce
both human and murine antibodies and will test these for preservation of antigen
binding. The Core will produce antibody that is validated for binding to DNA, DWEYS
and the extracellular domains of GluN2A/B. The Core will generate GluN2A/B
extracellular domains for use in ELISAs to confirm antibody specificity. Projects 2 and
3 requires that serum be tested for DNRAb titer. The Core will provide quality control
for these assays also. Project 3 will need large amounts of decoy antigen of high purity
to be produced.
This facility will be cost-effective and will maintain quality control for all 3 projects in the
Program. This represents an efficient approach to the generation of reagents to be
used by multiple investigators and one that maximizes the production of high quality
reagents.
Antibodies represent a highly versatile tool in medical research. They are used in
diagnostics, in basic and clinical research and in developing new therapies. The
DNRAbs created in our laboratory are utilized in all of the above areas not only in our
studies but in many laboratories within the United States and abroad.

## Key facts

- **NIH application ID:** 10454328
- **Project number:** 5P01AI073693-13
- **Recipient organization:** FEINSTEIN INSTITUTE FOR MEDICAL RESEARCH
- **Principal Investigator:** Czeslawa Helena Kowal
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $295,999
- **Award type:** 5
- **Project period:** 2008-08-01 → 2025-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10454328

## Citation

> US National Institutes of Health, RePORTER application 10454328, Core B- Monoclonal  Antibody and Reagent Core (5P01AI073693-13). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10454328. Licensed CC0.

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