# Dynamics of skin sensory specialization during vertebrate organogenesis

> **NIH NIH R01** · UNIVERSITY OF WASHINGTON · 2022 · $337,990

## Abstract

PROJECT SUMMARY/ABSTRACT
Organ development and function requires that neurons establish precise cellular interactions with tissue­resident
support cells. For example, touch­sensing somatosensory neurons project peripheral axons to the skin, where
they interact with specialized skin cell types. These specialized skin cells regulate axon development and modu­
late neuronal responses to cutaneous stimuli. Reciprocally, somatosensory neurons influence skin homeostasis.
Although the anatomy of vertebrate skin is well described, little is known about the dynamic process of sensory
specialization of the skin, partly because most studies have focused on mammalian embryos, which has limited
access to live­imaging.
The external development and the availability of unique transgenic tools make zebrafish an ideal model for study­
ing the dynamics of neuron and tissue maturation. This proposal investigates the development of a novel popula­
tion of specialized sensory cells that we identified in the zebrafish epidermis. Preliminary cellular, molecular and
developmental analyses suggest that these zebrafish epidermal cells are the equivalent of mammalian Merkel
cells, specialized mechanosensory cells that detect touch. The experiments proposed here investigate how de­
velopment of these specialized epidermal cells is coordinated with skin and nervous system maturation. In Aim
1, we will use live­imaging and genetic manipulation to characterize how sensory cell addition occurs during skin
growth. Aim 2 investigates the establishment of interactions between axons and epidermal cells and how neurons
promote skin specialization. Finally, in Aim 3, we will use in vivo photoconversion, lineage tracing and molecular
techniques to track the trajectory of skin­resident stem cells as they differentiate into sensory cells. Collectively,
these studies will provide mechanistic insights into organ specialization during development, interactions between
peripheral axons and their target tissues, and potentially point to the origins of touch­sensing disorders.

## Key facts

- **NIH application ID:** 10454376
- **Project number:** 5R01HD107108-02
- **Recipient organization:** UNIVERSITY OF WASHINGTON
- **Principal Investigator:** Jeffrey Philip Rasmussen
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $337,990
- **Award type:** 5
- **Project period:** 2021-09-01 → 2026-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10454376

## Citation

> US National Institutes of Health, RePORTER application 10454376, Dynamics of skin sensory specialization during vertebrate organogenesis (5R01HD107108-02). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10454376. Licensed CC0.

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