Project Summary. The Vision Center Core at Washington University serves a large and diverse group of clinicians and basic scientists studying the biology and pathology of the visual system. In partnership with the Department of Ophthalmology & Visual Sciences, the Vision Center Core will achieve three main goals. First, it will provide NEI-funded researchers (and others directly involved in vision science) with access to state-of-the-art instrumentation and technical support to enhance their individual research efforts. Second, it will enhance the environment for vision research at Washington University by fostering collaborations and attracting talented scientists to the field. Third, the Center Core will support the career development of the next generation of vision scientists. These goals will be achieved by provision of four Resource Cores, supported by an Administrative Core. Together they will provide the following services: 1. An Imaging Core will provide technical support in the preparation of ocular tissue for anatomical analysis. The core supports immunocytochemistry and in situ hybridization. Core investigators have ready access to confocal and multiphoton microscopes and OCT imaging. 2. A Visual Function Testing (VFT) Core will provide expertise, instrumentation, and training on equipment used to quantify visual performance in mice and other model systems. ERG, VEP and optomotry analysis are supported. 3. A Biostatistics & Bioinformatics Core (BBC) will provide statistical and methodological expertise in study design, assuring the validity of statistical analyses and reported results. Support is now provided for analysis of large data sets, such as those generated in the course of genomic or RNA sequencing experiments. 4. A Molecular Genetics Core will provide customized services for the production of transgenic and knockout mice using CRISPR/Cas9 technology. Assistance is also provided with design and production of gene targeting constructs, viral vectors, IVF services, and sperm/embryo cryopreservation. Provision of these support services and resources will greatly enhance the research capabilities of investigators at Washington University and facilitate collaboration among new and established vision scientists.