# Point-of-Care Microfluidic Biochip for Biomarkers Monitoring for Contributing in Early Sepsis Diagnosis

> **NIH NIH R01** · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · 2022 · $509,960

## Abstract

Principal Investigator/Program Director (Last, first, middle): Bashir, Rashid
Project Summary: Sepsis, a life-threatening organ dysfunction caused by a dysregulated host response to infection (Sepsis-
3 definition), is the leading cause of death and most expensive condition in hospitals. Annually, > 30 million people affected
worldwide, with at least 1.7 million adults developing sepsis (nearly 270K die) at a cost of $24 billion per year in the U.S.
Patients diagnosed with sepsis and no ongoing sign of organ failure have about a 15-30% chance of death. However, the
mortality rate can increase up to 40-60% for severe sepsis or septic shock patients. One in three patients who die in a hospital
have sepsis. One major factor in these rising mortality rates is the inability to accurately and quickly diagnose potentially
septic patients. Likewise, sepsis is a leading cause of hospital readmission (higher proportion than hospitalizations for heart
attack, heart failure, COPD, and pneumonia in the U.S.). EDs and ICUs rely on monitoring extremely non-specific
parameters (e.g. fever, low blood pressure, increased heart rate) to initiate a clinical diagnosis and begin treatment. These
crude indicators cause doctors to mistake early stage sepsis with several other diseases. A positive diagnose of early onset
sepsis is critical because mortality increases with delays in treatment. Survival rates have been reported to drop by 7.6%
every hour that the proper antibiotics are not administered, and these delays compound unnecessary hospital costs. Over the
last 30 years, clinics have used different criteria such as SIRS, LODS and SOFA or qSOFA as screening tools to assess the
severity of organ dysfunction in a potentially septic patient. Common factors among these criteria are non-specificity and
very high false positive rates. For patients with positive criteria, the final diagnostic test is a blood culture that may take up
to 5 day for a negative result. Likewise, blood culture has a very high false negative rate (> 60%) and does not work for
fastidious pathogens such as Chlamydia pneumoniae. More importantly, blood culture cannot be a gold standard method
for sepsis diagnosis. This technique only detects the presence of bacteria in the bloodstream (bacteremia), which does not
necessarily indicate illness. Many non-bacteremic infections can also cause life-threatening sepsis. In order to improve the
accuracy and sensitivity of sepsis diagnosis, the Sepsis-3 definition underscores the requirements for both pathogen
detection and information about the personalized state of the immune system of the patient. Therefore, we propose to focus
our efforts on monitoring selective biomarkers of this immune response. However, no single, or even a combination of
biomarkers has been validated for the diagnosis of sepsis. Because no single biomarker is specific enough to predict sepsis,
we propose to develop a point-of-care microfluidic biochip for measuring cell-surface and p...

## Key facts

- **NIH application ID:** 10462484
- **Project number:** 5R01AI148385-02
- **Recipient organization:** UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
- **Principal Investigator:** Rashid Bashir
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $509,960
- **Award type:** 5
- **Project period:** 2021-08-05 → 2025-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10462484

## Citation

> US National Institutes of Health, RePORTER application 10462484, Point-of-Care Microfluidic Biochip for Biomarkers Monitoring for Contributing in Early Sepsis Diagnosis (5R01AI148385-02). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10462484. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*