# Perivascular Fibroblast-Endothelium Interactions in Hypertension and Cerebral Ischemia

> **NIH NIH R21** · UNIVERSITY OF VIRGINIA · 2022 · $242,250

## Abstract

PROJECT SUMMARY (Description)
Perivascular fibroblasts (PVFs) and vascular endothelium are important responders to hypertension and
cerebral ischemia. Long-term aberrant interactions of PVFs and endothelium may also promote vascular
cognitive disorders (VCD), but the mechanisms remain poorly understood. This project will use PVF-specific
single-cell RNA-Seq and vascular endothelium-specific or TRAP-Seq to test the impacts of hypertension and
cerebral ischemia on gene expression. Outcomes of this project may suggest novel therapeutic targets.
Aim 1 will test the transcriptome changes of endothelial cells in a mouse model of chronic hypertension
(ReninTg) with and without partial cerebral ischemia.
Aim 2 will use bitransgenic ReninTg and Pdgfra-H2BGFP mice, with and without partial cerebral ischemia, to
isolate PVFs for single-cell RNA-Seq comparison.
In summary, this project will use open-ended genomic methods to correlate, for the first time, the transcriptome
adaptations by PVFs and vascular endothelium to hypertension and combined hypertension/ischemia.

## Key facts

- **NIH application ID:** 10463370
- **Project number:** 1R21NS127392-01
- **Recipient organization:** UNIVERSITY OF VIRGINIA
- **Principal Investigator:** Chia-Yi Kuan
- **Activity code:** R21 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $242,250
- **Award type:** 1
- **Project period:** 2022-04-01 → 2024-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10463370

## Citation

> US National Institutes of Health, RePORTER application 10463370, Perivascular Fibroblast-Endothelium Interactions in Hypertension and Cerebral Ischemia (1R21NS127392-01). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10463370. Licensed CC0.

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