Abstract (30 lines max): The purpose of this R13 submission is to request funds for a limited number of junior invited speakers and trainees from groups traditionally underrepresented to participate in the 2022 FASEB Science Research Conference on “Biological Methylation”. This will be the 14th FASEB SRC of the series and will be held at the Catania Sheraton Conference Center from June 19-23, 2022. The last FASEB meeting was held in Florence, Italy in 2018, where Dr. Guccione and Dr. Schneider were elected as co-chairs to work jointly with Dr. Davies and Dr. Ge who will be the two co-chairs for 2022. The conference was originally scheduled for 2020 and then cancelled due to the pandemic. The 2022 conference builds upon the scientific excellence of previous meetings, bringing together a diverse group of developmental and cancer biologists, chemists and computational biologists, both clinicians and basic scientists, from academia and pharma. The venue and scientific program will foster interaction between participants with different expertise and at different stages of their career. Previous evaluations have ranked this conference highly for outstanding presentations, discussion time, collegial atmosphere fostering mentorship and for the high impact speakers, who remain for the entire duration of the conference. In addition, we commit to an increased number of abstracts selected from trainees and will provide a platform for short presentations, poster sessions and sufficient free-time to foster collaborations. The 2022 program covers all major areas of high interest in the field of biological methylation such as lysine and arginine methylation, CpG methylation and novel RNA methylations. These are particular relevant fields of research given the recent promising clinical studies on several small molecule inhibitors in cancer therapy. Other sessions will deal with the structure and function of RNA, DNA and protein methyltransferases/demethylases, and protein post-translational methylation. The attendees’ background is very diverse, giving a unique opportunity to participants to interact with one another to further advance the basic understanding of biological methylation and to understand how methyltransferases and demethylases may be therapeutically targeted. In response to evaluation requests we have added several “meet the experts sessions” to discuss relevant issues such as “women in science” and “mentorship and transition to independence”. In addition, we will organize a Mini-symposium on “Small Molecule Inhibitors of methyltransferases and Demethylases”. To summarize, the specific goals of this conference are to: 1) provide a forum for the presentation of unpublished, cutting-edge research by investigators from diverse fields applicable to biological methylation, both from academia and pharma; 2) provide an opportunity for scientists studying diverse aspects of the biochemistry, function and disease relevance of SAM-dependent methylation, to d...