# CRCNS: Multiple Time Scale Memory Consolidation in Neural Networks

> **NIH NIH R01** · NEW YORK UNIVERSITY · 2022 · $395,986

## Abstract

Detailed description of the proposed use of the animals, including species,
strains, ages, sex, and number to be used;
Dissociated, primary cultures will be prepared from the cortex of new born mice of either
sex (mus musculus, Postnatal day 0-1). These experiments will be performed using
pups obtained from Vglut1-IRES2-Cre strains mated with Floxopatch (Lou et al., 2016)
strains so that pyramidal cells will express channel rhodopsin CheRiff and voltage
indicator QuasR2. Up to 5 cultures can be grown, which can be used for 5 experiments.
Justification for the use of animals, choice of species, and numbers to be used;
Description of procedures for minimizing discomfort, distress, pain, and injury;
and Method of euthanasia and the reasons for its selection.
Transgenic mice (category C) will be used because the experiments rely on the
expression of CheRiff and QuasR2 for optical stimulation/recording. There are no
alternative species.
 All experiments are terminal. Newborn mice will be cooled in ice prior to cervical
dislocation and excision of the brain for culture preparation. All procedures are in
accordance with guideleines of the NYU Animal Welfare Committee.
 The estimated number of animals below ensure that we have a continuous supply of
cultures to perform experiments on a daily basis. Based on our experience in the past 3
years, each postnatal day 0-1 (P0-P1) mouse will generate at least 5 culture
preparations. A viable culture will have a have a high density of neurons that express
the Floxopatch construct, which varies from mouse to mouse. Thus, if one culture is not
viable, all cultures derived from that mouse are also unusable. The success rate is
approximately 50%. Because cultures can be made only form P0-1 day old mice and
because it is not possible to determine whether expression is sufficient until the day of
the experiment 2-3 weeks later, we will need to make 2 sets of cultures per week. Thus,
we will need to maintain 2 breeding pairs per week or 8 breeding pairs per month to
have a continuous supply of cultures. The breeding pairs will either consist of wildtype
mice (for viral injection) or Vglut1-IRES2-Cre - Gt(ROSA)26Sor (floxopatch) mice.
Breeding pairs will be replaced every six months.
Total animals for experiments: 32 mice/year (=16 mice (8 breeding pairs) x 2/year
replacement of breeding pairs).
To maintain the 3 lines, 2 males and 2 females each of Vglut1-IRES2-Cre,
Gt(ROSA)26Sor, and wildtype mice will be kept in separate cages (4+4+4=12 mice
total). Every 6 months (twice per year), the mice of each type will be bred. From their
offsprings, 4 males and 4 females from each line will be weaned and kept in separated
cages as above (4+ 4 + 4 = 12) and the 4 original breeding pairs (12 mice) euthanized.
Overall, 24 mice/year will be used to maintain the lines.
Total mice/year: 56 (=24 mice/year to maintain lines + 32 mice/year for experiment).
Total mice over 3 years = 168 mice.
Reference
Lou et al., (2016) Genetically Targ...

## Key facts

- **NIH application ID:** 10468270
- **Project number:** 5R01MH129031-02
- **Recipient organization:** NEW YORK UNIVERSITY
- **Principal Investigator:** Stefano Fusi
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $395,986
- **Award type:** 5
- **Project period:** 2021-08-11 → 2024-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10468270

## Citation

> US National Institutes of Health, RePORTER application 10468270, CRCNS: Multiple Time Scale Memory Consolidation in Neural Networks (5R01MH129031-02). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/10468270. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*