# Proteomics and Macromolecular Crystallography Shared Resource

> **NIH NIH P30** · COLUMBIA UNIVERSITY HEALTH SCIENCES · 2022 · $223,657

## Abstract

PROTEOMICS AND MACROMOLECULAR CRYSTALLOGRAPHY SHARED RESOURCE: PROJECT 
SUMMARY 
The Herbert Irving Comprehensive Cancer Center (HICCC) Proteomics and Macromolecular Crystallography 
Shared Resource (PMCSR) provides access to advanced proteomic technologies and structural information 
of macromolecules (i.e. protein, DNA, RNA) at the atomic resolution for HICCC researchers. Directed by 
Rajesh Soni, PhD, the PMCSR services include: (1) protein Identification (gel band and section), (2) 
quantitative proteomics of affinity enriched protein complexes (IP, BioID and APEX2), (3) global quantitative 
proteomics analysis (Label free (LFQ) & Label (SILAC & TMT), (4) quantitative and targeted proteomics of 
cell signaling pathways (Immuno-affinity enrichment); v) exosome proteomics profiling, (5) targeted 
protein/peptide characterization/quantification (customized PRM), (6) structural modeling of proteins, (7) 
crystal structure of proteins, at atomic resolution in an apo form and in complex with natural ligands or 
inhibitors, and in complex with other macromolecules. These comprehensive and integrated services and 
technologies facilitate the quantitative, structural, and functional analyses of proteins implicated in cancer 
studied by HICCC members. The Macromolecular Crystallography service was established in 2015 with 
support from the HICCC and integrated into the existing Proteomics Shared Resource with the goal of 
expanding the capabilities and portfolio of services provided by the shared resource. Due to its advanced 
equipment, highly qualified staff, broad portfolio of specialized services, easy accessibility, and high cost- 
effectiveness, the PMCSR is the service provider of choice for HICCC investigators for proteomics and 
macromolecular crystallography services. The PMCSR provides customized services that are of central 
importance to the research of HICCC investigators and not easily accessible at other sites or from commercial 
suppliers. Consulting services in experimental design are conducted by expert personnel in the PMCSR who 
facilitate the incorporation of advanced techniques into the research portfolio of HICCC investigators. The 
PMCSR will continue to incorporate novel proteomic and structural biology technologies, to provide state-of- 
the-art services, and to promote high value consultation with the goal of empowering the research programs 
at the HICCC. During the current project period (2014-2019), the PMCSR was utilized by 50 HICCC members, 
supported key data and insights facilitating 13 peer-reviewed publications including five journals with an 
impact factor >20 (Nature Medicine, Cancer Cell, Cancer Discovery), and currently supports research in 20 
NIH-funded research project grants (11 from NCI).

## Key facts

- **NIH application ID:** 10469552
- **Project number:** 5P30CA013696-47
- **Recipient organization:** COLUMBIA UNIVERSITY HEALTH SCIENCES
- **Principal Investigator:** Brent R. Stockwell
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $223,657
- **Award type:** 5
- **Project period:** 1997-07-04 → 2025-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10469552

## Citation

> US National Institutes of Health, RePORTER application 10469552, Proteomics and Macromolecular Crystallography Shared Resource (5P30CA013696-47). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10469552. Licensed CC0.

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