# Flow and Tissue Cytometry Core

> **NIH NIH U54** · INDIANA UNIVERSITY INDIANAPOLIS · 2022 · $209,077

## Abstract

ABSTRACT
A quantitative Flow and Tissue Cytometry core (FTCC, core 3) is proposed for the Cooperative Center of
Excellence in Hematology (CCEH) to support the research efforts of members of the center. Flow cytometry is
an integral research tool for the vast majority of CCEH investigators. Of the 25 current CCEH members, 16
routinely utilize classical flow cytometric services in their research, and more than 65% of their publications
over the last 10 years utilized flow cytometric services. However, many need to interpret flow cytometry
information in the context of the spatial relationships in the hematopoietic niche; for example, to identify how
cells interact with one another and with cellular and physical components of the niche in normal and stressed
conditions. To address this need, the FTCC will complement flow cytometry with unique imaging services,
including 3D tissue cytometry and intravital microscopy. In this way, the core will offer CCEH members a
critical service along with the opportunity to extend their interrogation of hematopoietic cells to include
quantitative analyses of the spatial distribution of HSC with other cells during different conditions of
nonmalignant hematopoiesis. The FTCC capitalizes on existing shared facilities and expertise present within
the Indiana University School of Medicine (IUSM) to provide CCEH members with the latest in technologies
and services expected form a sophisticated flow and tissue cytometry facility. The FTCC will provide a cost-
effective resource to CCEH investigators who can utilize this core to apply a powerful and integrated approach
to studies of non-malignant hematology that will be an invaluable asset to their research, as well as to
hematology investigators across the country. The specific aims of the FTCC are: 1- Provide outstanding,
consistent, timely, and economical multicolor analysis, cell sorting, image flow analysis, and single cell
proteomic analysis to all CCEH members to facilitate the conduct of their research in hematopoiesis and stem
cell biology that requires any of these services. 2- Enhance the productivity of CCEH members by providing
centralized and standardized flow cytometric and other related services that allow for the exchange of
protocols and results between investigators within and outside Indiana University and advance the distribution
of scientific knowledge. 3-Provide CCEH members with large-scale 3D and highly-multiplexed 2D tissue
cytometry services, extending from study design, fluorescence labeling, image collection, quantitative image
analysis and supervised and unsupervised data analysis. 4- Provide CCEH members with intravital microscopy
services, including preparation of institutional compliance documents, study design, animal surgery, image
collection and quantitative image analysis. This arrangement will give CCEH investigators and all investigators
Nationwide, powerful new tools for their nonmalignant hematology investigations.

## Key facts

- **NIH application ID:** 10473860
- **Project number:** 5U54DK106846-07
- **Recipient organization:** INDIANA UNIVERSITY INDIANAPOLIS
- **Principal Investigator:** Edward F Srour
- **Activity code:** U54 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $209,077
- **Award type:** 5
- **Project period:** 2015-08-01 → 2026-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10473860

## Citation

> US National Institutes of Health, RePORTER application 10473860, Flow and Tissue Cytometry Core (5U54DK106846-07). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10473860. Licensed CC0.

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