# The Interplay of Electric Potential and Morphology of Biomembranes

> **NIH NIH R01** · NORTHWESTERN UNIVERSITY · 2022 · $306,797

## Abstract

An electric potential difference across the plasma membrane is common to all living cells and is crucial for
the generation of action potentials for cell-to-cell communication. Beyond excitable nerve and muscle,
bioelectric signals conjugated with the transmembrane potential control many cell behaviors such as
migration, orientation, and proliferation, which play crucial role in embryogenesis, would healing, and cancer
progression. The mechanisms of cellular responses to electric stimuli are virtually unknown. An electricitycentered view, epitomized by the Hodgkin-Huxley model, focuses on the voltage-dependent ion channels.
However, in recent years membrane mechanics is emerging as a potentially important player: membrane
deformations are detected to co-propagate with action potentials, several ion channels have been found to
be both voltage-gated and mechanosensitive, and lipid rafts have been implicated as electrosensors.
Assessment of the relevance of these membrane-related effects in bioelectric phenomena requires
fundamental understanding of the coupling between membrane morphology, stresses, and voltage, which is
limited.
To fill this void, the team proposes a combined theoretical and experimental study of biomimetic
membranes with transmembrane potential induced by an externally applied electric fields. Specifically, the
project seeks to determine how an electric potential elicits membrane responses such a stretching or
compression, curvature, and phase transitions, and vice versa, how changes in the membrane morphology
modulate the transmembrane potential. Mathematically, these are challenging free boundary problems
exhibiting complex dynamics. Continuum theory will be used to model the ions transport, motion of a charged
lipid membrane interface and the surrounding liquids. A computational method is proposed to solve these
complicated transient three-dimensional free-boundary problems. Experimentally, using giant unilamellar
vesicles (GUVs) as a model membrane system the PI will develop novel methodologies to probe the dynamic
coupling between shape and voltage of biomembranes. The techniques will be based on the flickering
spectroscopy (analysis of the thermally driven micron- and sub-micron membrane undulations) and GUV
deformation in applied electric fields. Membranes with broad range of compositions mimicking biological
membranes will be investigated. The experimental results will inform the mathematical models in terms of
relevant physics and material parameters, and vice versa, the theories will guide the experiments.

## Key facts

- **NIH application ID:** 10476459
- **Project number:** 5R01GM140461-03
- **Recipient organization:** NORTHWESTERN UNIVERSITY
- **Principal Investigator:** Petia M Vlahovska
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $306,797
- **Award type:** 5
- **Project period:** 2020-09-05 → 2024-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10476459

## Citation

> US National Institutes of Health, RePORTER application 10476459, The Interplay of Electric Potential and Morphology of Biomembranes (5R01GM140461-03). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10476459. Licensed CC0.

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