# Genome Editing Shared Resource

> **NIH NIH P30** · VANDERBILT UNIVERSITY MEDICAL CENTER · 2022 · $171,743

## Abstract

CORE 008 – GENOME EDITING SHARED RESOURCE
PROJECT SUMMARY/ABSTRACT
The mission of the Genome Editing Shared Resource (GESR), previously the Transgenic Mouse/Embryonic
Stem Cell Shared Resource, is to enable the Vanderbilt-Ingram Cancer Center (VICC) research community,
inclusive of our VICC members and partners at Meharry Medical College, Tennessee State University, and other
NCI Cancer Centers to efficiently generate, store and share genetically-altered mouse models. GESR, which
has been in existence for over 25 years, has undergone major changes over the past five years. Five prior
service lines oriented around gene targeting in mouse embryonic stem cells (mESCs) became unnecessary after
the discovery of CRISPR/Cas9. Thus, in late 2015, GESR shifted its focus towards improving the efficiency and
outcomes of CRISPR/Cas9-mediated gene editing in one and two cell mouse embryos. Since then, over 800
mutant mice from 97 CRISPR/Cas9 experiments have been generated, with a 100% technical success rate for
the past twenty-six projects. GESR now provides a full-service approach where the resource performs project
design, pre-injection reagent validation, post-injection analysis of the resulting pups, and breeding of the first
generation to provide the investigator with a correctly edited heterozygous mouse. Investigators no longer need
extensive molecular biology skills, or even much knowledge about CRISPR/Cas9 gene editing strategies. To
support the sharing of these new CRISPR-generated lines, GESR has become more proactive in encouraging
the use of core cryopreservation services. In house cryopreservation of mouse lines improves colony
management and enables specific pathogen free stocks to be sent as cryopreserved germplasm to other
institutions. The resource continues to reconstitute cryopreserved mouse lines that are requested by VICC
investigators from other investigators, or that exist within external repositories, by performing both in vitro
fertilization and embryo transfer. Finally, the resource has established, and are striving to expand, a Vanderbilt
Cryopreserved Mouse Repository (VCMR). The VCMR facilitates compliance with NIH Mouse Sharing Policies
and enables storage and distribution of mice from Vanderbilt without the continued involvement of investigators.
All microinjection and embryo transfer services are performed in a specific pathogen free barrier facility.
Together, the combination of services provided by the GESR enables VICC members and other Vanderbilt
investigators to efficiently generate and maintain novel genome-edited mouse models to study multiple aspects
of cell and tumor biology using sophisticated mouse models.

## Key facts

- **NIH application ID:** 10488749
- **Project number:** 5P30CA068485-27
- **Recipient organization:** VANDERBILT UNIVERSITY MEDICAL CENTER
- **Principal Investigator:** MARK A MAGNUSON
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $171,743
- **Award type:** 5
- **Project period:** 1998-09-01 → 2025-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10488749

## Citation

> US National Institutes of Health, RePORTER application 10488749, Genome Editing Shared Resource (5P30CA068485-27). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10488749. Licensed CC0.

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