# Proteome-wide assessment of the impact of citrullination on the activityof transcription factors in Th2 cells

> **NIH NIH R21** · BRIGHAM AND WOMEN'S HOSPITAL · 2022 · $215,050

## Abstract

Project Summary
Many proteins are modified after they are produced in cells. Such modification can critically influence the
function of proteins. One such modification is citrullination, which has been shown to regulate the function of
immune cells, including lymphocytes and neutrophils. Abnormal citrullination has been associated with many
human diseases, including infection and autoimmune diseases. Thus, manipulating protein citrullination can be
beneficial in many clinical settings; however, our knowledge of citrullination is still very limited mainly due to
lack of reliable methods for examining the functional impact of citrullination. The objective of this project is to
develop a method that can be used to assess the proteome-wide impact of citrullination on the activity of
transcriptional regulators in immune cells. A DNA-proteomic platform will be optimized and subsequently used
to compare the activity of transcriptional regulators at a proteomic scale between citrullination sufficient and
deficient lymphocytes. The approach can be readily modified and applied to other immune cells. Results thus
generated very likely will lead to discoveries of novel therapeutic targets of human diseases.

## Key facts

- **NIH application ID:** 10493375
- **Project number:** 5R21AI165967-02
- **Recipient organization:** BRIGHAM AND WOMEN'S HOSPITAL
- **Principal Investigator:** I-CHENG HO
- **Activity code:** R21 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $215,050
- **Award type:** 5
- **Project period:** 2021-09-22 → 2024-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10493375

## Citation

> US National Institutes of Health, RePORTER application 10493375, Proteome-wide assessment of the impact of citrullination on the activityof transcription factors in Th2 cells (5R21AI165967-02). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10493375. Licensed CC0.

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