# Genomic/Genetic and Proteome

> **NIH NIH P01** · UNIVERSITY OF ARIZONA · 2022 · $231,922

## Abstract

ABSTRACT:
The Genome and Proteome Core (PPG Core B) will provide essential, cutting-edge genomics, proteomics, and
bioinformatics expertise to facilitate the translational impact of this highly integrated PPG. Core B will be respon-
sible for providing the research investigators with: (i) expert interrogation of publicly available DNA and RNA
sequencing datasets and genome-wide DNA methylome datasets to generate interpretable data from these plat-
forms; (ii) genetic expertise needed to test for associations between PPG target gene DNA variants and sus-
ceptibility and severity of ARDS; (iii) generation of human endothelial cell (EC) lines and genome-edited cell line
models with selected single nucleotide knock in or deletion-based knock out mutations for functional genetic
analyses; (iv) execution of traditional proteomic mass spectrometry analysis of immunoprecipitated PPG target
proteins for protein translational modification (PTM) identification, including phosphorylation, nitration, and ubiq-
uitination; (v) in silico computational protein-structure modeling to identify the impact of functional non-synony-
mous coding variants on protein structure and function (protein-protein interaction, catalytic activity, post-trans-
lational modification), with protein-protein interactions validated by surface plasmon resonance; and, (vi) exper-
tise to oversee production of recombinant proteins with mutations at sites of PTMs or SNPs. As a centralized
core for all genome and proteome analyses, Core B (located in Tucson AZ) will perform all relevant analysis
of experimental samples for all four projects as each PPG Project will require the services provided by
Core B.

## Key facts

- **NIH application ID:** 10496457
- **Project number:** 2P01HL126609-06A1
- **Recipient organization:** UNIVERSITY OF ARIZONA
- **Principal Investigator:** Nathan A. Ellis
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $231,922
- **Award type:** 2
- **Project period:** 2016-09-20 → 2023-01-05

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10496457

## Citation

> US National Institutes of Health, RePORTER application 10496457, Genomic/Genetic and Proteome (2P01HL126609-06A1). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/10496457. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*