In autoimmune endocrine disease, self-reactive T cells inappropriately target hormone-producing cells, leading to tissue damage and obliteration of hormone production. One of the most common autoimmune diseases, Hashimoto’s thyroiditis, offers an ideal model to decipher how hormone production is lost in autoimmune endocrine disease. Surprisingly, only 20-40% of patients with Hashimoto’s thyroiditis exhibit loss of hormone production (hypothyroid). The remainder of patients retain hormone production despite immune infiltration of the thyroid (euthyroid). Understanding how hormone production is preserved requires dissection of interactions between infiltrating T cells and hormone-producing epithelial cells, such as thyrocytes. To address this challenge, I have performed single-cell RNA sequencing in human thyroid from patients with Hashimoto’s thyroiditis, Graves’ disease (an autoantibody-driven form of thyroid autoimmunity) and those without autoimmune thyroid disease. We have identified a transcriptionally unique population of thyrocytes that is significantly expanded in Hashimoto’s thyroiditis and ectopically expresses class II MHC. In addition, our preliminary data indicate several thyroid-infiltrating CD4+ T cell populations that appear to distinguish Graves’ disease from Hashimoto’s thyroiditis. Epithelial cells are known to express MHCII in inflamed tissue, however it is not known whether these cells present antigen to CD4+ T cells in vivo. We hypothesize that MHCII+ thyrocytes present antigen to and modulate the phenotype of CD4+ T cells, leading to global changes in the inflammatory environment. We further hypothesize that the transcriptional phenotype of MHCII+ thyrocytes differs in euthyroid and hypothyroid Hashimoto’s thyroiditis. To test these hypotheses, I will compare the transcriptional and functional phenotype of MHCII+ thyrocytes in euthyroid and hypothyroid Hashimoto’s thyroiditis and Graves’ disease (Aim 1). I will then determine how thyroid-infiltrating self-reactive T cells differ among these conditions (Aim 2). This project may define how tissue function can be preserved in autoimmune disease more broadly. This proposal presents a five-year plan for Dr. Michelle Rengarajan to train in immunology to decipher how epithelial-immune interactions underly endocrine autoimmunity. Dr. Rengarajan will be an Instructor in Medicine at Harvard Medical School (HMS) and Massachusetts General Hospital (MGH). She will perform the proposed work under mentorship from Drs. Andrew Luster and Alexandra-Chloe Villani. Dr. Rengarajan has outlined a career development plan focused on single-cell genomics, epithelial-immune interactions, antigen-specific T cells, with additional training in scientific communication and leadership. Dr. Rengarajan’s long-term goal is to develop an independent research program studying the mechanistic basis of autoimmune endocrinopathies. The experiments and training plan outlined in this proposal, with the collaborativ...