# CHEETAH Center for the Structural Biology of HIV Infection, Restriction, and Viral Dynamics

> **NIH NIH U54** · UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH · 2022 · $373,611

## Abstract

CORE SUMMARY
This Core is designed to provide CHEETAH Center members with access to novel approaches for imaging and measuring
the behaviors of different HIV-host systems under investigation in our Center. Specifically, our Tools for Structural and
Mechanistic Studies Core (Core 2), will develop new methodology and provide CHEETAH Center members with access to
state-of-the-art instrumentation and expertise in Fluorescence Spectroscopy and Dynamics, Scaffolds for Structure
Determination, and Electron Cryotomography.
Component 1 (Fluorescence Spectroscopy and Dynamics) will provide instrumentation and expertise in fluorescence
labeling and single-molecule fluorescence approaches to studies of biological structure and dynamics. Novel methods for
correlating biological processes in real time and for linking cryoEM structural studies to dynamic analyses are also being
developed.
Component 2 (Scaffolds for Structure Determination) will generate and provide antibody-like molecules called “Synthetic
Antigen Binders” (sABs) as fiducial markers for cryoEM structure determinations. The Component will also optimize and
apply new, empowering classes of “universal” molecular fiducials based on: 1) transportable binding epitopes, or 2)
secondary antibodies. Universal fiducials for more challenging RNAs and RNA-protein complexes are also being created.
Component 3 (Electron Cryotomography) will incorporate the powerful and rapidly advancing technologies of correlative
light and electron microscopy (CLEM), focused ion beam (FIB) milling, and electron cryotomography (ECT) to reconstruct
native molecular landscapes at unprecedented resolution. The Component will also develop new functional correlative
reporters that can be located by fluorescence or by electron energy-loss spectroscopy (EELS) imaging, and apply these
reporters to problems in HIV-1 sub-viral organization, virus entry, and viral core replication and trafficking.

## Key facts

- **NIH application ID:** 10508315
- **Project number:** 1U54AI170856-01
- **Recipient organization:** UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH
- **Principal Investigator:** JOSEPH D PUGLISI
- **Activity code:** U54 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $373,611
- **Award type:** 1
- **Project period:** 2022-07-11 → 2027-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10508315

## Citation

> US National Institutes of Health, RePORTER application 10508315, CHEETAH Center for the Structural Biology of HIV Infection, Restriction, and Viral Dynamics (1U54AI170856-01). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10508315. Licensed CC0.

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