The current application seeks continued salary support for Hans Minderman, PhD who serves as the Associate Director of the Flow and Image Cytometry Shared Resource (FICSR) of the Roswell Park Comprehensive Cancer Center (Roswell Park). This position was created in 2007 to oversee the research applications of the various imaging (confocal, live cell imaging) and flow cytometry platforms available in the facility and to apply his extensive experience in these areas in collaborations and consultations with the FICSR investigator user base. With over 35 years of experience in clinical and research applications of flow and image cytometry (30 years at Roswell Park), Dr Minderman throughout his career has collaborated with many investigators at the Cancer Center on NIH funded projects. The FICSR is an integral part of Roswell Park’s Cancer Center Support grant (P30CA016056) for which the cancer center Director, Dr Johnson serves as the Principal Investigator. The Tumor Immunology & Immunotherapy (TII) CCSG program, previously co-led by the Unit Director on the original application, Dr Odunsi, is the major user base of the FICSR; but numerous NIH-funded researchers across all 5 CCSG programs actively collaborate and consult with Dr Minderman commensurate with the purpose of the PAR 21-286 funding opportunity. During his first R50 tenure (2016-2021), Dr Minderman’s contributions to Roswell Parks research enterprise have been significant and impactful specifically with regards to the NIH-funded research. As of October 2021, 137 extramural grant applications dependent on FICSR were awarded to Roswell Park faculty, 59 were awarded by NIH, 22 of which to members of the TII program. The cancer center has also made significant investments in the FICSR with the acquisition of a new confocal microscope, two spectral flow cytometry analyzers and a 3rd Fortessa analyzer. With the changes in the R50 program now making the distinction between Laboratory (PAR-20-288)- and Core (PAR-20-287)-based scientists and with the FICSR being an integral part of the Roswell Park CCSG infrastructure, Dr Johnson as the PI of the CCSG grant, will now serve as the Unit Director for the renewal application.