# Neuroendocrine control of synaptic connectivity.

> **NIH NIH R01** · ALBERT EINSTEIN COLLEGE OF MEDICINE · 2022 · $512,073

## Abstract

PI: Buelow, Hannes E.
 Project Summary
 The general body plan of most animals follows a bilateral symmetry. Some organs such as the heart and
liver break this gross anatomical symmetry, while other structures such as the brain display a superficial
bilaterally symmetric anatomy. Nonetheless, it has been known for a long time that the two hemispheres of the
human brain serve distinct functions, and many classical examples in neuroscience and psychology have
shown the importance of asymmetry in brain function. For example, higher order cognitive abilities such as
language, spatial orientation, attention, and visual processing exhibit left-right (L-R) functional asymmetries in
humans. Of note, many neuropsychiatric conditions including autism spectrum disorders, depression,
schizophrenia, and post-traumatic stress disorder display defects in brain laterality, further underscoring the
importance of lateralized brain function. Not surprisingly, neuropsychiatric conditions often have a genetic and,
hence possibly, a developmental component. Most of these conditions are also influenced by environmental
factors, yet how the environment interfaces with connectivity remains largely unknown. We have identified an
asymmetric synaptic connection between two pairs of sensory neurons in the nematode Caenorhabditis
elegans that changes in response to experience. Importantly, this connection is controlled cell-non-
autonomously from other cells by insulin signaling, which in turn is regulated by experience. This provides a
paradigm to investigate, on a molecular level and in single cell resolution, how the environment can change
hardwiring of a neural circuit in an experience-dependent manner. The goal of this proposal is to investigate
the developmental, plastic and functional aspects of this connection using C. elegans as a model system. In
Specific Aim 1, we will determine the mechanisms by which experience changes connectivity. We will
determine whether transcription or translation is required and whether neuronal activity is necessary and
sufficient, and in which cells. In Specific Aim 2, we will determine the role of insulin signaling in controlling
synaptic connectivity. Specifically, we will test which insulin-like agonists and antagonists function in which
cells to effect the changes in connectivity; where the receptor functions and in which genetic context. Lastly, in
Specific Aim 3, we will determine how changes in connectivity translate into changes in information flow and
behavior using whole brain calcium imaging and behavioral experiments. In sum, our research program aims
to establish the mechanisms, by which the environment changes synaptic hardwiring and behavior in the
context of an asymmetric synaptic connection.

## Key facts

- **NIH application ID:** 10522227
- **Project number:** 1R01NS125134-01A1
- **Recipient organization:** ALBERT EINSTEIN COLLEGE OF MEDICINE
- **Principal Investigator:** Hannes Erich Buelow
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $512,073
- **Award type:** 1
- **Project period:** 2022-05-15 → 2027-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10522227

## Citation

> US National Institutes of Health, RePORTER application 10522227, Neuroendocrine control of synaptic connectivity. (1R01NS125134-01A1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10522227. Licensed CC0.

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