Abstract The Case Transgenic and Targeting Facility is the sole transgenic core serving NIH-funded researchers in Northeast Ohio. The core has created and cryopreserved mouse genetic models for over twenty years. These mouse models have been used by local researchers and researchers around the world to study cancer, cystic fibrosis, neurodegeneration, viral immunology, skeletogenesis, cardiovascular disease, and many other diseases and fundamental biological processes. Gene editing increased the number of models that the core has been able to create. Now more than ever, the NIH-funded researchers we serve need these strains backed up, archived for future retrieval, or distributed to their collaborators and other scientists as cryopreserved sperm or embryos. This application requests funding for equipment which will modernize the core's mouse strain cryopreservation, make it more cost-efficient, increase capacity and bring the operation in line with current best practices. The core needs this modernization to meet the demand from local researchers, research centers, and from new institutional research initiatives in translation of gene-directed therapies and in studies of the microbiome.