PROJECT SUMMARY/ABSTRACT This application by the Biorepository and Tissue Technology Shared Resource (BTTSR) requests funding for a BioStore™ M60 Cryo-190°C liquid nitrogen (LN 2) system to support ongoing collection of human solid biospecimen and long-term storage for future research, a goal in line with those of the NCI-designated comprehensive Moores Cancer Center (MCC), at the University of California, San Diego. This automated BioStore M60 Cryo system is a cryogenic storage unit that offers sample storage automation, cold chain management, and improved security and accessibility while maintaining sample protection within a liquid nitrogen environment. The requested instrument goes beyond conventional manual LN2 Freezers with the highest sample protection, comprehensive inventory management systems, superior user safety measures, and LIMS connectivity. The BioStore™ M60 Cryo -190°C System provides sample integrity, security and tracking with availability of Full 21-CFR-11 reporting capability. Adequate preservation of biospecimens has been proven to be critical to obtain reliable and reproducible results in genomics, transcriptomics, proteomics, developing PDX models, immunoprofiling and many other assays. Biological assays which depend on -190°C stored biospecimens can be extremely sensitive to transient temperature exposure thus the superior quality control measures of this system are needed. The new BioStore™ M60 Cryo -190°C system will ensure that our users have access to high quality human biospecimens, and the automation of the system will also enhance the integrity of samples during storage and retrieval by minimizing transient temperature exposure. The BioStore™ M60 Cryo -190°C system will be located, administered and maintained in the existing BTTSR facility at the MCC.