# Automation of Liquid Nitrogen Freezer for Cryopreservation of Unique Human Biospecimens

> **NIH NIH R24** · UNIVERSITY OF CALIFORNIA, SAN DIEGO · 2022 · $292,290

## Abstract

PROJECT SUMMARY/ABSTRACT
This application by the Biorepository and Tissue Technology Shared Resource (BTTSR) requests funding for a
BioStore™ M60 Cryo-190°C liquid nitrogen (LN 2) system to support ongoing collection of human solid
biospecimen and long-term storage for future research, a goal in line with those of the NCI-designated
comprehensive Moores Cancer Center (MCC), at the University of California, San Diego. This automated
BioStore M60 Cryo system is a cryogenic storage unit that offers sample storage automation, cold chain
management, and improved security and accessibility while maintaining sample protection within a liquid
nitrogen environment. The requested instrument goes beyond conventional manual LN2 Freezers with the
highest sample protection, comprehensive inventory management systems, superior user safety measures, and
LIMS connectivity. The BioStore™ M60 Cryo -190°C System provides sample integrity, security and tracking
with availability of Full 21-CFR-11 reporting capability.
Adequate preservation of biospecimens has been proven to be critical to obtain reliable and reproducible results
in genomics, transcriptomics, proteomics, developing PDX models, immunoprofiling and many other assays.
Biological assays which depend on -190°C stored biospecimens can be extremely sensitive to transient
temperature exposure thus the superior quality control measures of this system are needed. The new BioStore™
M60 Cryo -190°C system will ensure that our users have access to high quality human biospecimens, and the
automation of the system will also enhance the integrity of samples during storage and retrieval by minimizing
transient temperature exposure. The BioStore™ M60 Cryo -190°C system will be located, administered and
maintained in the existing BTTSR facility at the MCC.

## Key facts

- **NIH application ID:** 10533708
- **Project number:** 1R24OD033752-01
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN DIEGO
- **Principal Investigator:** Alfredo Alberto Molinolo
- **Activity code:** R24 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $292,290
- **Award type:** 1
- **Project period:** 2022-08-15 → 2024-02-14

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10533708

## Citation

> US National Institutes of Health, RePORTER application 10533708, Automation of Liquid Nitrogen Freezer for Cryopreservation of Unique Human Biospecimens (1R24OD033752-01). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/10533708. Licensed CC0.

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