# The impact of Cannabis on inflammation and HIV-1 reservoirs in Zambia (Supplement 2)

> **NIH NIH R01** · LSU HEALTH SCIENCES CENTER · 2022 · $672,259

## Abstract

Abstract
 Factors, including co-morbidities, chronic inflammation, and immune activation are known to affect HIV-1
reservoirs and persistent infection. The impact of recreational drugs such as cannabis in HIV-1 patients
remains understudied particularly in the African setting where HIV-1 is epidemic. A majority of the individuals
infected with HIV-1 currently live in Africa where poverty, high HIV-1 infection rates and increasing rates of
substance abuse co-exist. In Zambia, a sub-Saharan country in the heart of the HIV-1 epidemic, high
prevalence cannabis use has been reported. Coupled with the high regional HIV-1 prevalence, the reports of
substance abuse suggest that there is a need to determine how lifestyle choices, such as use of cannabis,
might impact inflammation, persistent HIV-1 replication, or viral reservoirs in anti-retroviral therapy (ART)
treated individuals. It has been shown that in aviremic ART treated individuals, low-level immune activation
persists and contributes to viral persistence. Cannabinoids have been shown to modulate immune activation
and inflammatory responses, and have demonstrated clinical impact in a number of chronic inflammatory
disorders. The anti-inflammatory properties of cannabinoids could reduce the cellular activation that drives
persistent low-level HIV-1 expression and therefore reduce the size of latent HIV pools. For these reasons,
investigation of potential positive or negative effects of cannabis on inflammation, latent HIV reservoirs, or on
HIV-1 replication in various tissues including the central nervous system is a highly relevant endeavor.
 We propose to leverage our existing HIV research infrastructure and expertise resulting from ongoing
collaborations in Zambia for this study. We will collect and analyze autopsy cases from Zambian HIV-1
positive, cannabis users and controls to test the hypothesis that the anti-inflammatory properties of cannabis
will lead to reduced persistent HIV-1 replication, in turn, leading to a reduction in the size and distribution of
latent viral reservoirs.   Our overall goals are to determine whether cannabis use correlates with reduce local
immune activation, altered size and distribution of HIV-1 tissue reservoirs or reduced levels of persistent viral
replication in those reservoirs. Our goal will be accomplished by addressing two specific aims: 1) Determine
the prevalence of tissue pathology, the level of inflammation and immune activation in the brain and other
potential tissue HIV-1 reservoirs, and define correlations with cannabis usage; 2) Determine whether cannabis
use impacts the level of persistent viral replication or the size, distribution, and cellular composition of latent
HIV-1 reservoirs in tissues. This study will lead to a greater understanding of the impact of cannabis usage
and its effects on inflammation, on the distribution, the size and maintenance of HIV-1 reservoirs. The use of
unique postmortem samples, supports the direct testing of a var...

## Key facts

- **NIH application ID:** 10533925
- **Project number:** 7R01DA044920-05
- **Recipient organization:** LSU HEALTH SCIENCES CENTER
- **Principal Investigator:** Charles Wood
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $672,259
- **Award type:** 7
- **Project period:** 2017-08-01 → 2025-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10533925

## Citation

> US National Institutes of Health, RePORTER application 10533925, The impact of Cannabis on inflammation and HIV-1 reservoirs in Zambia (Supplement 2) (7R01DA044920-05). Retrieved via AI Analytics 2026-05-27 from https://api.ai-analytics.org/grant/nih/10533925. Licensed CC0.

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