PROJECT SUMMARY The COVID-19 pandemic is undergoing a new wave of infections in the US fueled by new, highly transmissible variants of concern and waning vaccine immunity. The need for more, rapid diagnostic tests is clear. In this proposal, we will build on the success of the Johns Hopkins POC Technologies Research Center for Sexually Transmitted Diseases which has been leveraged to support the Rapid Acceleration of Diagnostics Technology Program. In this supplemental grant proposal, we have 4 aims: 1) to add an international COVID-19 clinical research site for diagnostic testing and biorepository development at the Infectious Diseases Institute in Kampala with the team with whom we have been collaborating for 2 cycles of POCTRN funding and where vaccination rates are low and there are on-going waves of infection; 2) to continue to convene the highly successful Clinical Review Committee for RADx Tech Wave 2 and add on desktop evaluations of in vitro devices; 3) to use next generation sequencing and microarrays to monitor variants and other respiratory pathogens that cause influenza-like illness; and 4) to provide support for specific companies needing technology development expertise and biospecimens to accelerate development.