# DNA-based assay for calorimetric determination and quantitation of protein concentrations in pure or mixed solutions

> **NIH NIH R43** · THERMOCAP LABORATORIES INC. · 2022 · $258,625

## Abstract

Abstract
An attractive feature of using in vitro expression systems to generate biological compounds is the
capability to produce a wide variety of compounds (proteins, peptides, antibodies) on various scales, in a
cheap and rapid manner. Current methods to analyze any of these produced compounds require
purification which poses a major drawback for working with products derived from expression systems.
For example, a moderately abundant (soluble) protein can require 27 individual steps over four days to
purify; a significant amount of time and expense to screen a single protein. Screening a library of potential
biological compounds for therapeutic value, requires many purification steps for each compound before
any analysis can be performed. This includes measurements as simple as determination of the mass of
the produced biological compounds. To this end we have developed a unique DNA-based scheme using
differential scanning calorimetry for determination of the masses of biological compounds in both purified
and mixed media. Uniquely, our probes are universally applicable to a wide range of biological compounds
enabling mass quantification prior to purification. This advance allows for initial analysis of biologics early
in the production process, lowering associated time requirements and resource costs. An added benefit
is the possibility to supplant the numerous mass analysis kits currently on the market that rely on
colorimetric or fluorescent methods. These methods are highly specific to the types of compounds
assayed and have limitations preventing universal applicability. Our product offers a superior advantage
of existing methods. In this project we plan to screen a library of biologically expressed SARS-CoV-2 spike
and nucleocapsid proteins generated by our commercial collaborator. The aim of this work is to evaluate
whether our assay is capable of predicting protein yields from their expression systems solely from a mass
determination of their unpurified samples. Additionally, we will use our assay to verify the protein
structure before and after purification.

## Key facts

- **NIH application ID:** 10547595
- **Project number:** 1R43GM146542-01A1
- **Recipient organization:** THERMOCAP LABORATORIES INC.
- **Principal Investigator:** Matthew Walter Eskew
- **Activity code:** R43 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $258,625
- **Award type:** 1
- **Project period:** 2022-09-06 → 2024-01-05

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10547595

## Citation

> US National Institutes of Health, RePORTER application 10547595, DNA-based assay for calorimetric determination and quantitation of protein concentrations in pure or mixed solutions (1R43GM146542-01A1). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10547595. Licensed CC0.

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