# Core-001

> **NIH NIH U19** · DUKE UNIVERSITY · 2024 · $677,572

## Abstract

The overall goal of the Animal Models Core is to support all programs of the IPCAVD by performing 
immunization studies in CH58 unmutated common ancestor (UCA) VH + VL knock-in (KI) mice and rhesus 
macaques, with ALVAC-C vCP2438 prime and modified mRNA encoding trivalent mosaic gp120s for induction 
of polyfunctional anti-HIV-1 non-neutralizing antibodies (NNAbs) (Project 1, Aims 2 and 3), and by overseeing 
toxicity studies using CGMP clinical trials material (CTM) mRNAs in collaboration with Project 2. This work will 
be facilitated by recent innovative advances made in a number of complementing areas, including the 
development of novel SHIV constructs expressing transmitted/founder HIV-1 envelopes, well-tolerated 
adjuvants that substantially increase the immunogenicity of HIV-1 envelope protein immunogens and induce T 
follicular helper cell (Tfh) responses, technology for reproducibly generating envelope-specific monoclonal 
antibodies from immunized macaques, and use of the new CH58 VH and VL knock-in mouse that expresses 
one of the NNAb ADCC-mediating antibodies from RV144, CH58 V2 antibody. The Specific Aims of this 
Animal Models Core will include: 
Aim 1. Perform immunization studies in CH58 unmutated common ancestor (UCA) homozygous 
(hom)/heterozygous (het) VH + VL knock-in (KI) mice ALVAC-C vCP2438 prime and modified mRNA 
encoding trivalent mosaic gp120s in Project 1, Aim 2. 
Aim 2. Perform immunization studies with ALVAC-C vCP2438 prime and boosting with and boosting 
with ALVAC-C plus modified mRNA encoding trivalent mosaic gp120s mRNAs in rhesus macaques 
(RMs) (Project 1, Aim 3). 
Aim 3. Perform immunization studies in CH58 UCA hom/het VH + VL KI mice to confirm CGMP clinical 
trials materials immunogenicity, and oversee toxicity studies of ALVAC-C + CGMP mRNAs in 
collaboration with Project 2.

## Key facts

- **NIH application ID:** 10547811
- **Project number:** 5U19AI142596-05
- **Recipient organization:** DUKE UNIVERSITY
- **Principal Investigator:** Barton F. Haynes
- **Activity code:** U19 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $677,572
- **Award type:** 5
- **Project period:** 2019-01-11 → 2025-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10547811

## Citation

> US National Institutes of Health, RePORTER application 10547811, Core-001 (5U19AI142596-05). Retrieved via AI Analytics 2026-05-29 from https://api.ai-analytics.org/grant/nih/10547811. Licensed CC0.

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