# A novel robotic wastewater analysis system to quantify opioid exposure and treatment in residential communities

> **NIH NIH R44** · BIOBOT ANALYTICS, INC. · 2022 · $174,937

## Abstract

ABSTRACT.
Illicit psychostimulant use is increasing across the United States leading to significant morbidity and mortality. A
2019 cross sectional study found that individuals with self reported methamphetamine use disorder in the US
has tripled since 2015. The downstream comorbidities associated with methamphetamine and other
psychostimulants like cocaine, ketamine and phencyclidine result in increased healthcare utilization,
medication nonadherence, infectious complications of drug use, and worsening mental health and trauma.
Despite its importance, surveillance of psychostimulant use remains suboptimal; most existing surveillance
strategies utilize large national self-report sampling or data from emergency department and substance use
disorder clinic urine drug screens resulting in significant bias. This surveillance data is utilized to inform
national public health priorities and on a community scale, allocation of resources to address substance use
disorders. Wastewater-based epidemiology is a potential option to conduct population level drug surveillance.
By measuring key parent compound and metabolites in wastewater networks, a prevalence estimate of drug
use and trends of use over time can be visualized. By varying the location of sample collection in the
wastewater network, substance use data can be described at a large wastewater treatment plant catchment
area or at the community level by measuring substances at individual street level manholes. Our ongoing fast
track SBIR leverages wastewater networks to develop and commercialize an opioid assay that measures both
parent opioids as well as key metabolites that indicate consumption. This administrative supplement iterates on
our successful parent grant by selecting four psychostimulants of

## Key facts

- **NIH application ID:** 10549579
- **Project number:** 3R44DA051106-03S1
- **Recipient organization:** BIOBOT ANALYTICS, INC.
- **Principal Investigator:** Peter R Chai
- **Activity code:** R44 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $174,937
- **Award type:** 3
- **Project period:** 2022-02-01 → 2023-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10549579

## Citation

> US National Institutes of Health, RePORTER application 10549579, A novel robotic wastewater analysis system to quantify opioid exposure and treatment in residential communities (3R44DA051106-03S1). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10549579. Licensed CC0.

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