# An Integrated In Vitro 3D Model of Human Bone Marrow and Peripheral Infection

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA AT DAVIS · 2022 · $12,404

## Abstract

PROJECT SUMMARY
The primary goal of the supplement to be carried out by Ms. Zarate-Sanchez is to design, build, and validate
an integrated “organ-on-a-chip” platform of human primary tumor and human bone marrow. This is important
because: 1) all tumors engage the immune system, and thus the bone marrow (primary source of immune
cells); and 2) a wide range of tumors co-opt the bone marrow to the advantage of the tumor during progression
and latency (e.g., breast cancer). The research milestones include: 1) demonstrate proficiency in all steps of
soft lithography, including CAD design, photolithography, and PDMS device construction; 2) demonstrate
proficiency in cell culture techniques; and loading and culture of in vitro bone marrow and cancer models in the
microfluidic device; 3) quantify number, phenotype, and location of the leukocytes within the microfluidic device
in response to a series of breast cancer cell lines; and 4) quantify number, phenotype, and location of a series
of breast cancer cells lines in the microfluidic device. In addition to the research milestones, the supplement
will seek to accomplish a series of milestones associated with her career development including: 1) completing
3 elective courses; 2) mentoring undergraduate researchers; 3) presenting her work at a national meeting; 4)
publishing her work in a peer-reviewed archived journal(s); and 5) submitting her own independent F31
application. Accomplishing these milestones will prepare Ms. Zarate-Sanchez for an independent and
impactful scientific career.

## Key facts

- **NIH application ID:** 10550076
- **Project number:** 3R01EB030410-01A1S1
- **Recipient organization:** UNIVERSITY OF CALIFORNIA AT DAVIS
- **Principal Investigator:** Steven CARL George
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $12,404
- **Award type:** 3
- **Project period:** 2022-04-08 → 2024-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10550076

## Citation

> US National Institutes of Health, RePORTER application 10550076, An Integrated In Vitro 3D Model of Human Bone Marrow and Peripheral Infection (3R01EB030410-01A1S1). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10550076. Licensed CC0.

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