# Epigenetic Regulation of Cortical Neuronal Lineage Progression

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · 2021 · $268,691

## Abstract

Cortical radial glia are neural stem cells that self renew and produce all cortical neuron cell types in an orderly
sequential fashion. There is a fundamental gap in understanding the molecular mechanism that underlies the
orderly production of neuronal cell types. Our overall goal is to understand the intrinsic timing mechanism that
regulates cell fate transitions during cortical neurogenesis. We have identified the transcriptional regulator
Prdm16 (Positive Regulatory Domain-containing 16) as being a critical component for regulating precisely
timed cell fate transitions during cortical neurogenesis. Our studies of Prdm16 serve as an entry point to
understanding the relevant genetic and epigenetic programs regulating the mode of radial glia cell division, and
its relationship to neuronal fate potential. In this proposal we plan to utilize MARIS (Method for Analyzing RNA
following Intracellular Sorting) to define the molecular programs regulated by Prdm16 in a cell type and stage
specific manner. We will also determine the temporal pattern of PRDM16 binding and regulation of cis-
regulatory elements during cortical neurogenesis. The finding from our studies will have a direct impact on
increasing our understanding of how expression of early transcriptional programs and chromatin modifications
can contribute to neurodevelopmental disorders.

## Key facts

- **NIH application ID:** 10570771
- **Project number:** 7R01NS102228-06
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
- **Principal Investigator:** COREY C HARWELL
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $268,691
- **Award type:** 7
- **Project period:** 2022-02-11 → 2023-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10570771

## Citation

> US National Institutes of Health, RePORTER application 10570771, Epigenetic Regulation of Cortical Neuronal Lineage Progression (7R01NS102228-06). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10570771. Licensed CC0.

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