# NCITU: National Center for In-situ Tomographic Ultramicroscopy. Supplement

> **NIH NIH U24** · NEW YORK STRUCTURAL BIOLOGY CENTER · 2022 · $242,444

## Abstract

Summary
The National Center for In-situ Tomographic Ultramicroscopy (NCITU) is based at the Simons Electron
Microscopy Center (SEMC), which is a part of the New York Structural Biology Center (NYSBC), based in New
York, NY. NCITU is dedicated to developing and providing access to both standard and advanced methods for
in-situ cryoET specimen preparation, and to disseminating this expertise through a cryoET cross-training
program designed to serve a wide variety of skill levels and career goals.
The primary goal of the National Center for In-situ Tomographic Ultramicroscopy (NCITU) is to provide user
access and service towards preparing specimen for direct visualization of macromolecular associations and
organization, particularly in native cells. A highly qualified technical team provides direct hands-on service,
guidance, and assistance to ensure that samples and grids are prepared with the highest quality and in the
shortest time possible. An already well established cross-training program is being expanded and extended to
provide training in every aspect of the cryoET specimen preparation pipeline.
This is a request for supplemental funding for the National Center for In-situ Tomographic Ultramicroscopy
(NCITU) to enhance the Center's data analysis and CLEM capabilities. This is a specific request for (i) a
dedicated cryoET processing workstation, (ii) a Zeiss LSM 900 software upgrade, and (iii) a second Linkam
CMS196 cryo CLEM stage.

## Key facts

- **NIH application ID:** 10579763
- **Project number:** 3U24GM139171-03S1
- **Recipient organization:** NEW YORK STRUCTURAL BIOLOGY CENTER
- **Principal Investigator:** BRIDGET Olivia CARRAGHER
- **Activity code:** U24 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $242,444
- **Award type:** 3
- **Project period:** 2020-09-15 → 2024-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10579763

## Citation

> US National Institutes of Health, RePORTER application 10579763, NCITU: National Center for In-situ Tomographic Ultramicroscopy. Supplement (3U24GM139171-03S1). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10579763. Licensed CC0.

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