# Sequencing and Epigenetics Core

> **NIH NIH U19** · LA JOLLA INSTITUTE FOR IMMUNOLOGY · 2023 · $187,043

## Abstract

Sequencing and Epigenetics Core (Core C)
Core Summary
The proposed projects in this CCHI program will use genetic approaches to define comprehensive molecular 
properties of specific immune cells in different aspects of immune responses in different diseases and after 
vaccination from blood and human clinical tissues (lymph nodes and lung tissue). Facing the inherent challenge, 
of cell abundance and heterogeneity when working with human samples, the CCHI Sequencing and Epigenetics 
Core, unlike any other external facility, will provide a unique and comprehensive set of procedures especially 
validated for isolating and processing small numbers of immune cell populations and support downstream single-cell and bulk transcriptomic as well as epigenetic studies. First, the centralized team of the Sequencing Core will
provide assistance to all CCHI project users for careful collection of small number of immune cells, adequate 
processing and storage of samples for all transcriptomic and epigenetic assays listed in the proposal. Second, 
the Sequencing Core will perform all micro-scaled bulk RNA-seq as well as single-cell RNA sequencing and will 
provide support for the whole transcriptome analysis, TCR and BCR highly variable sequence extraction from 
bulk and single-cell RNA-seq datasets. Finally, the Sequencing Core will perform micro-scaled library preparation
for epigenetic assays (ATAC- and ChIP-Seq).

## Key facts

- **NIH application ID:** 10580760
- **Project number:** 5U19AI142742-05
- **Recipient organization:** LA JOLLA INSTITUTE FOR IMMUNOLOGY
- **Principal Investigator:** Gregory Seumois
- **Activity code:** U19 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2023
- **Award amount:** $187,043
- **Award type:** 5
- **Project period:** 2019-03-11 → 2024-02-29

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10580760

## Citation

> US National Institutes of Health, RePORTER application 10580760, Sequencing and Epigenetics Core (5U19AI142742-05). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10580760. Licensed CC0.

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