# Calcium coding mechanisms in plant cell growth and immunity

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA BERKELEY · 2022 · $66,680

## Abstract

This equipment supplement application seeks funding for replacement of a broken growth
chamber for culturing plant materials used in the parent project. The model plant Arabidopsis
must be grown and maintained in a controlled environments. In particular, our plants are
routinely transgenic containing foreign DNAs and considered “genetically modified
organisms” (GMOs) and thus must be contained in a specified chamber. We are also working
with pathogenic bacteria that induce immune responses in plants. Such pathogens also
require containment. We have been using the Conviron plant growth chambers for these
critical purposes. We had two old growth chambers (27 years) at the beginning of the parent
project. An accidental floor flooding and leak above the growth chamber facility occurred and
one of the growth chambers was severely damaged and we requested funding through the
same supplement program in 2021 to replace it. In February 2022, the other old chamber
failed due to old age. Two growth chambers are minimally required to grow plants with and
without pathogen treatments. I thus request funding herein to purchase a new chamber to
replace the failed chamber to ensure steady progress of the project and reduce the chance
of cross-contamination of plant materials.
 To ensure the growth chamber operates correctly and are maintained properly, we
propose to purchase the growth chamber from the same company Conviron so that our lab
is familiar with the use of the chamber. Our University greenhouse and facility staff team will
commit to the preventive maintenance of the growth chamber. We expect to have the new
chamber in use as early as August 2022 should the funding become available in July. The
two new growth chambers will provide a solid foundation for supplying plant materials in this
funding period and future renewal of the project.

## Key facts

- **NIH application ID:** 10581257
- **Project number:** 3R01GM138401-03S1
- **Recipient organization:** UNIVERSITY OF CALIFORNIA BERKELEY
- **Principal Investigator:** Sheng Luan
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $66,680
- **Award type:** 3
- **Project period:** 2020-09-01 → 2024-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10581257

## Citation

> US National Institutes of Health, RePORTER application 10581257, Calcium coding mechanisms in plant cell growth and immunity (3R01GM138401-03S1). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/10581257. Licensed CC0.

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