# Equipment supplement for NCDIR

> **NIH NIH P41** · ROCKEFELLER UNIVERSITY · 2022 · $102,790

## Abstract

PROJECT SUMMARY
The National Center for Dynamic Interactome Research.
The emergent properties of life require the dynamic interactions of macromolecules, the two major classes of
which are proteins and nucleic acids. These interactions form macromolecular machines and dynamic liaisons
that shape the cell, transmit information and control cellular behaviors, and pathogenic alterations in molecular
interaction networks (interactomes) underlie disease. Our understanding and modulation of biological systems,
as well as their pathologies, thus relies on the ability to elucidate and interpret these interactions and their
dynamics. For nucleic acids, recent advances have led to an explosion of genomic data. However, proteins are
incredibly diverse in their abundance and their properties, making them highly versatile for their dynamic tasks,
but at the same time exceptionally difficult to analyze. It is for these reasons that the interactomic revolution still
lags very far behind the genomic revolution.
The National Center for Dynamic Interactome Research (NCDIR) couples cell biology laboratories, a mass
spectrometry laboratory, a systems biology laboratory, and a computational structural biology laboratory. The
goal of the NCDIR is to synergistically pioneer new and improved approaches, integrating these technologies
into a fundamentally unique “pipeline” approach to address the urgent need of the biomedical community for
technologies that can rapidly, reliably and routinely identify and characterize the dynamic cellular interactome.
Our pipeline approach begins by developing technologies for purifying and preserving, with high fidelity, various
defined forms of the hierarchical arrangement of interactors surrounding any chosen macromolecule. We then
provide comprehensive, highly quantitative, detailed temporal and structural data for dynamic complexes. Such
data is used to generate structural and mechanistic models that are predictive, testable, actionable, and guide
experiments to focus on those that are most informative. The models aim to provide the biomedical community
with the means for rational target-based future experimentation, functional characterizations and even
intervention. These approaches will be developed, refined and beta-tested via a selected set of Driving Biological
Projects and Collaborations that can enter and exit our pipeline at any point, and which present specific
technological challenges. Critical to the design of the NCDIR is an effective training and dissemination program
that is responsive to the urgent needs of the biomedical community.

## Key facts

- **NIH application ID:** 10581258
- **Project number:** 3P41GM109824-09S1
- **Recipient organization:** ROCKEFELLER UNIVERSITY
- **Principal Investigator:** MICHAEL P ROUT
- **Activity code:** P41 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $102,790
- **Award type:** 3
- **Project period:** 2014-08-01 → 2024-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10581258

## Citation

> US National Institutes of Health, RePORTER application 10581258, Equipment supplement for NCDIR (3P41GM109824-09S1). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10581258. Licensed CC0.

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