The goal of this administrative supplement request is to provide needed upgrades to a Nikon spinning disc confocal microscope screening for studies on mitochondrial fission proteins for R01GM067180. Altered mitochondrial fission has severe consequences even death. Yet the reasons for this are unknown. It is postulated that the mitochondria have their own lifecycle that involves fission of unhealthy mitochondria to remove them. In this model, the proper balance of fission is critical: either excess or impaired fission result in unhealthy mitochondria. This model is compelling because it explains how alterations in fission can cause or contribute to many fundamentally different diseases including recovery from heart attack and stroke, increased metabolic stress from diabetes, normal aging, and neurodegenerative diseases such as Parkinson's and Alzheimer's disease. To determine this, we have generated several mutants, many of which are pathological. We are testing hypotheses by determining how these mutants impact biomolecular interactions inside cells. The requested instrumentation will greatly accelerate our work as it allows for faster data collection and analysis including the use of cutting- edge machine learning tools. Detailed microscopic information will provide a better understanding of the protein machinery and how it works, which will identify key points of regulation that may be targeted with small molecules to inhibit, and activate, fission. The discovery of such molecules may ultimately lead to treatments for diseases in which enhanced, or impaired, fission is central.