# BCCMA:Foundational Research to Act Upon and Resist Conditions unfavorable to bone (FRACTURECURB):Role of abaloparatide for fracture healing

> **NIH VA I01** · VETERANS AFFAIRS MED CTR SAN FRANCISCO · 2023 · —

## Abstract

The guiding research strategy of the collaborative projects is to use pre-clinical models of disease that weaken
bone or delay fracture repair of high relevance to the VA population, employing in vivo/in vitro models to
identify ‘druggable’ mechanisms enabling the testing of promising therapies directed at those mechanisms, while
incorporating best methods and outcome measures utilizing the combined expertise of the group. Fractures,
and in particular delayed or non union of fractures, is a major cause of morbidity and lost productivity in our
Veterans. Better treatment is required. Parathyroid hormone (PTH) treatment shows promise in accelerating
fracture repair and closing non unions. Our previous studies demonstrated that IGF1 signaling is required for the
anabolic actions of PTH, and that the actions of IGF1 are in turn dependent on ephrinB2/EphB4 bidirectional
signaling. Abaloparatide is an analog of PTH that appears even more anabolic to bone than PTH. But how these
anabolic agents actually promote fracture repair is unclear, and the fracture repair process is itself complex. We
have focused on three sites at which fracture repair takes place within the callus in fractured long bones, each
with unique characteristics. 1) Intramembranous bone formation occurs on the periosteal surface of the fractured
cortex involving direct differentiation of periosteal osteoprogenitors to osteoblasts. 2) Endochondral bone
formation bridges the gap between the broken ends of bone involving differentiation of periosteal osteochondroal
progenitors into chondrocytes which then transdifferentiate into osteoblasts forming the bony callus. 3)
Intramedullary bone formation occurs within the marrow of the broken bone pieces initiated by a unique set of
osteocytes and potentially transcortical vessel perivascular cells expressing stem cell markers that move from
the cortex into the marrow to differentiate (dedifferentiate) into osteoblasts forming bone within the marrow. We
hypothesize that abaloparatide, acting on these different cells, all of which express the PTH/PTHrP receptor
through which abaloparatide acts, will promote the coordinated repair process via mechanisms that include IGF1
regulated ephrinB2/EphB4 coupling between the osteoprogenitors, chondrocytes, osteoblasts, and perivascular
cells essential for fracture repair. We will use a variety of mechanical, molecular, and imaging means established
in our own laboratory and that of our collaborators in the collaborative VAMR proposal to examine the response
to abaloparatide at each of these sites. We will then test this response in mouse models in which the IGF1
receptor and ephrinB2 have been deleted from the cells involved with fracture repair to test the role of these
signaling mechanisms in the response to abaloparatide. Our project has three aims. Aim 1 will demonstrate
whether abaloparatide accelerates fracture repair via IGF1/ephrinB2/EphB4 signaling at each site of
regenerative bone formation by me...

## Key facts

- **NIH application ID:** 10584445
- **Project number:** 1I01BX005854-01A2
- **Recipient organization:** VETERANS AFFAIRS MED CTR SAN FRANCISCO
- **Principal Investigator:** DANIEL David BIKLE
- **Activity code:** I01 (R01, R21, SBIR, etc.)
- **Funding institute:** VA
- **Fiscal year:** 2023
- **Award amount:** —
- **Award type:** 1
- **Project period:** 2022-10-01 → 2026-09-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10584445

## Citation

> US National Institutes of Health, RePORTER application 10584445, BCCMA:Foundational Research to Act Upon and Resist Conditions unfavorable to bone (FRACTURECURB):Role of abaloparatide for fracture healing (1I01BX005854-01A2). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10584445. Licensed CC0.

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