# Supplement for Resource for Quantitative Elemental Mapping for the Life Sciences

> **NIH NIH P41** · MICHIGAN STATE UNIVERSITY · 2022 · $100,000

## Abstract

PROJECT SUMMARY
Inorganic chemistry plays myriad, evolutionarily conserved roles in physiology and pathology. Cells must
accumulate several metals, such as zinc and iron, to millimolar levels to survive. They can deploy fluctuations in
metal content to control processes as varied as the mammalian cell cycle, pathogen infection and neurological
function. The critical regulatory role of metals is emphasized by the observation that one-third of all protein-
encoding genes in the human genome encode metal-dependent proteins. There is an increasing appreciation in
the NIH research community that intracellular content and subcellular location of each element provides an
inorganic signature that serves as a quantitative phenotype. These realizations are driving the demand for new
technologies for quantitative evaluation of inorganic signatures in cells and tissues. Such methods are essential
to understanding the regulation of physiological and pathogenic processes and developmental decisions. The
proposed Resource for Elemental Imaging for Life Sciences (QE-Map) will develop and integrate emerging
technologies to create transformative approaches to the compelling biological question concerning inorganic
chemistry in health and disease.
The technologies to be developed comprise a suite of three imaging and detection methods that will allow
investigators to quantitatively map the distribution of dozens of elements in samples ranging from cell extracts
to fixed cells to tissue slices. The complementary and integrative nature of these methods is critical to enabling
investigators to examine fluxes in intracellular ion content and localization, and to link these fluxes to changes in
distribution within tissues and in living animals. A multi-disciplinary team, located at Northwestern University and
Argonne National Laboratories, will address current limitations of LA-ICP-MS and SXFM technologies and will
launch the development of photoacoustic methods and probes to enable studies at the tissue level. We will
develop workflows and software that allows co-registration of images and standardization of quantitative data
that will maximize the impact and accelerate application to a broad range of biomedical research.
A portfolio of twelve DBPs was selected for their capacity to enable iterative development of new methods and
address high impact research questions in the field of “inorganic physiology.” The DBPs focus on 4 themes: (a)
metal regulation in brain function and pathology; (b) metal modulation of host-pathogen interactions; (c) metal
fluxes controlling reproduction and development; and (d) metal imbalances in metabolic pathology.
A Community Engagement program will foster training of new technology users and dissemination of the
technologies to the scientific community. The integration and coordination of Resource projects and activities
will be enabled by the Administrative Core, co-directed by Drs. Thomas O’Halloran and Chris Jacobsen, and
supported by...

## Key facts

- **NIH application ID:** 10586510
- **Project number:** 3P41GM135018-03S1
- **Recipient organization:** MICHIGAN STATE UNIVERSITY
- **Principal Investigator:** Chris Johnson Jacobsen
- **Activity code:** P41 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $100,000
- **Award type:** 3
- **Project period:** 2020-07-01 → 2025-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10586510

## Citation

> US National Institutes of Health, RePORTER application 10586510, Supplement for Resource for Quantitative Elemental Mapping for the Life Sciences (3P41GM135018-03S1). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10586510. Licensed CC0.

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