# Recombinant HIV-1 Env glycoprotein immunogens and antibodies production and structural characterization

> **NIH NIH P01** · FRED HUTCHINSON CANCER CENTER · 2022 · $248,149

## Abstract

PROJECT SUMMARY/ABSTRACT
 Broadly neutralizing antibodies are likely to be an important component of an HIV-1 vaccine. The HIV-1
Envelope (Env) glycoprotein is the sole target of neutralizing antibodies and has been a focus of immunogen
designs. Yet diverse recombinant (rec) Envs do not display detectable binding to the inferred germline (iGL) of
certain bNAbs, such as the VRC01-class bNAbs, which are the focus of our HIVRAD project. Rec Envs capable
of binding iGL VRC01 bNAbs have been designed and induced the production of iGL VRC01 bNAbs when used
as immunogens. However these novel immunogens are not sufficient to induce the maturation of iGL VRC01-
class bNAbs to their broad neutralization forms. In this HIVRAD, we propose to use novel reagents as
immunogens to guide the development of VRC01-class bNAbs. The Reagents and Structural Core will provide
high quality reagents to Projects 1-3 of this HIVRAD as well as structural information on all antibodies described
and obtained in this HIVRAD (vaccine-elicited or from naïve humans or animals) in complex with their epitopes.
This information will help us to better understand the B cell response elicited by our novel immunogens and will
assist us in improving our “prime-boost” schemes.

## Key facts

- **NIH application ID:** 10593445
- **Project number:** 6P01AI138212-05
- **Recipient organization:** FRED HUTCHINSON CANCER CENTER
- **Principal Investigator:** Marie Pancera
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $248,149
- **Award type:** 6
- **Project period:** 2018-12-06 → 2023-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10593445

## Citation

> US National Institutes of Health, RePORTER application 10593445, Recombinant HIV-1 Env glycoprotein immunogens and antibodies production and structural characterization (6P01AI138212-05). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10593445. Licensed CC0.

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