# INTERCEPT: Integrated Research Center for human Pain Tissues

> **NIH NIH U19** · WASHINGTON UNIVERSITY · 2022 · $2,347,608

## Abstract

Abstract: Tens of millions of Americans suffer from chronic pain. Opioids represent the main tool for
treating pain, but their use in chronic pain conditions suffers from a poor evidence base and the inherent risk of
addiction. The current crisis of opioid-related deaths highlights the risk associated with widespread opioid use.
The PRECISION Human Pain Network, part of the NIH HEAL Initiative, seeks to provide foundational data on
the diversity among cell types that comprise the pain neuraxis. Here, we propose a U19 program entitled
“Integrated Research Center for Human Pain Tissues” (INTERCEPT Pain). We will build on prior successes in
our human tissue research program, and expand the scope of work to directly address the goals of the
PRECISION Human Pain Network. Our program leverages the world-class genomics and computational assets
of the McDonnell Genome Institute here at Washington University and substantial institutional strengths in
neurobiology of pain, axon degeneration and regeneration, and genetics in a coordinated program to develop
foundational new knowledge regarding the transcriptional and functional properties of tissues and cells involved
in pain transduction, transmission and modulation in humans. We will provide a comprehensive atlas of cellular
gene expression in human peripheral nerve using single nuclei RNAseq, spatial transcriptomics and multiplex
proteomics, and leverage this to understand the distribution of genes involved in traumatic (neuromas) and
idiopathic (hereditary) painful neuropathies. We will also provide expanded single-nucleus sequencing atlases
of dorsal root ganglia, including DRG from donors without and with a recent history of pain. We will generate a
spatial atlas of hDRG using imaging mass cytometry, and optimize computational approaches for integrated IMC
and single cell transcriptomic analysis of hDRG. Finally, we will combine analysis of electrophysiological,
transcriptional, and morphological data from hDRG and human spinal cord dorsal horn neurons. We will work
with other centers to optimize protocols and data collection to allow integrated analysis across multiple centers,
in collaboration with the U24 DCIC and HEAL data ecosystem. These goals will be accomplished through the
coordinated activity of 3 scientific projects, led by international leaders in the fields of pain neurobiology, genetics,
and mechanisms of axon degeneration and regeneration. The project titles are as follows:
Project 1: Multi-omics peripheral nerve atlas enables fine-mapping of pain molecular phenotypes.
Project 2: Characterization of the human dorsal root ganglia at the single cell level via integrated transcriptomics
and spatial proteomics
Project 3: Functional and genetic characterization of human DRG and spinal cord at single cell resolution
The projects are supported by an experienced program leadership team and an administration core, a human
tissue procurement and processing core, and a data core.

## Key facts

- **NIH application ID:** 10593843
- **Project number:** 1U19NS130607-01
- **Recipient organization:** WASHINGTON UNIVERSITY
- **Principal Investigator:** Robert W Gereau
- **Activity code:** U19 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $2,347,608
- **Award type:** 1
- **Project period:** 2022-09-30 → 2027-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10593843

## Citation

> US National Institutes of Health, RePORTER application 10593843, INTERCEPT: Integrated Research Center for human Pain Tissues (1U19NS130607-01). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10593843. Licensed CC0.

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